| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 83,853 | A→C | 48.2% | I237I (ATA→ATC) | ftsW → | integral membrane protein involved in stabilising FstZ ring during cell division |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 83,853 | 0 | A | C | 48.2% | ‑3.6 / 99.0 | 81 | I237I (ATA→ATC) | ftsW | integral membrane protein involved in stabilising FstZ ring during cell division |
| Reads supporting (aligned to +/- strand): ref base A (17/25); new base C (18/21); total (35/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCC > minE/83792‑83912 | cGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCg < 1:2237772/69‑1 (MQ=255) gAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGa < 1:701378/69‑1 (MQ=255) gAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGa < 1:1456784/69‑1 (MQ=255) gAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGa < 1:1236373/69‑1 (MQ=255) gAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGa < 1:769251/69‑1 (MQ=255) aaaTTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGa < 1:2965113/68‑1 (MQ=255) aTTGCCATTATCGGTATTGGCATTTCAGCGGTTGTGTTGCTGATCCTCGc > 1:1953765/1‑50 (MQ=255) aTTGCCATTATCGGTATGGGGATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:97300/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:3311185/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:256709/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:709608/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:1273771/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:2157824/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:3353944/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:2135541/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:2621414/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:1694388/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:1030667/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:2099973/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:17804/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:366192/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccgc > 1:2831272/1‑69 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccg > 1:1088743/1‑68 (MQ=255) aTTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATCCTCGCCGAACCGTACCGTATccg > 1:836036/1‑68 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:2650890/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:2511996/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:2298511/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:3231679/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:1757532/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:1739896/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:687742/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:1450448/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:1236789/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:1098594/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCgtgt < 1:1038032/65‑1 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTAc > 1:1826778/1‑68 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTAc > 1:1825767/1‑68 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTAc > 1:2113486/1‑68 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTAc > 1:2187931/1‑68 (MQ=255) aTCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTAc > 1:978764/1‑68 (MQ=255) ggTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCa > 1:49663/1‑69 (MQ=255) ggTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCa > 1:2265279/1‑69 (MQ=255) aTGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTc > 1:2775796/1‑69 (MQ=255) aTGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTc > 1:1520839/1‑69 (MQ=255) aTGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTc > 1:3109122/1‑69 (MQ=255) aTGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTc > 1:1561848/1‑69 (MQ=255) aTGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTc > 1:3211888/1‑69 (MQ=255) ggCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAaccgtaccg > 1:2572286/1‑44 (MQ=255) ggCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAaccgtaccg > 1:1076254/1‑44 (MQ=255) ggCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAaccgtaccg > 1:1817003/1‑44 (MQ=255) ggCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAaccgtaccg > 1:1650013/1‑44 (MQ=255) ggCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAaccgtaccg > 1:621506/1‑44 (MQ=255) tgtgTTGCTGATCCTCGCCTAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTTGGaa < 1:574519/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTTGGaa < 1:3069546/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:1910905/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:333017/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:355019/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:69186/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:1229667/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:828284/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:1208498/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:969727/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:1031222/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:2197236/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:2264098/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:2119027/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:3300585/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:2110491/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:2898302/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:3210991/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:3097855/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:3330393/67‑1 (MQ=255) tgtgTTGCTGATCCTCGCAGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGaa < 1:2992163/67‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:1264658/68‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:202576/68‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:209914/68‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:92473/68‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:3267134/68‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:1918078/68‑1 (MQ=255) ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:338128/68‑1 (MQ=255) tGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATcc < 1:2022544/67‑1 (MQ=255) | CGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCC > minE/83792‑83912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |