| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 901,754 | T→C | 49.2% | H533H (CAT→CAC) | ycjM → | predicted glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 901,754 | 0 | T | C | 49.2% | ‑6.0 / 57.5 | 63 | H533H (CAT→CAC) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (11/21); new base C (23/8); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAG > minE/901689‑901817 | gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:2767140/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:2970557/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:632094/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:621361/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:2504374/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:248278/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:2355216/68‑1 (MQ=255)gaTTGCCTGACGGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACGGt < 1:3192213/68‑1 (MQ=255) tGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTg < 1:2238664/69‑1 (MQ=255) aTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGtt < 1:2214911/68‑1 (MQ=255) aTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGtt < 1:2800305/68‑1 (MQ=255) aTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGtt < 1:2361878/68‑1 (MQ=255) aTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGtt < 1:310300/68‑1 (MQ=255) aTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGtt < 1:757945/68‑1 (MQ=255) gTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2140810/68‑1 (MQ=255) gTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:1279660/68‑1 (MQ=255) gTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2107999/68‑1 (MQ=255) gTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:468743/68‑1 (MQ=255) tCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGata < 1:2490092/69‑1 (MQ=255) tAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATAtt > 1:2138323/1‑69 (MQ=255) tAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATAtt > 1:3374020/1‑69 (MQ=255) atatTCAGCATGTAAATATTACTAATCTGCATGGTc > 1:2313648/1‑36 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:3093881/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2987952/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2562351/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2515097/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2425350/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:3264944/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:3120753/49‑1 (MQ=255) aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:310523/49‑1 (MQ=255) tAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGc > 1:3283373/1‑69 (MQ=255) ttACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGcc < 1:934214/69‑1 (MQ=255) tAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa < 1:2211920/36‑1 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATAtt > 1:2691237/3‑40 (MQ=39) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGaaa > 1:1527028/3‑51 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1066125/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1221955/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:895393/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:793803/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1447342/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1607437/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:589573/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:534996/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:165580/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:43712/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:43007/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1715803/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:3273683/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:17264/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:3229838/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1753706/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:3157689/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:1951514/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:2153422/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:2283261/3‑69 (MQ=255) accTGCACGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACACTGCATCCCTGGc > 1:2770915/3‑69 (MQ=255) cTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAg > 1:1663130/1‑69 (MQ=255) tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGcc > 1:3314990/1‑60 (MQ=255) tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGcc > 1:40391/1‑60 (MQ=255) tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAg > 1:425124/1‑68 (MQ=255) tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAg > 1:2377647/1‑68 (MQ=255) tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAg > 1:305546/1‑68 (MQ=255) tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAg > 1:2655209/1‑68 (MQ=255) | AATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAG > minE/901689‑901817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |