| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 905,541 | T→C | 52.9% | D213D (GAT→GAC) | ycjQ → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 905,541 | 0 | T | C | 52.9% | 12.1 / 89.5 | 104 | D213D (GAT→GAC) | ycjQ | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (27/22); new base C (15/40); total (42/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGG > minE/905480‑905606 | aTCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa < 1:365916/69‑1 (MQ=255) gCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa > 1:1348136/1‑66 (MQ=255) gCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa > 1:1759077/1‑66 (MQ=255) gCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa > 1:1560798/1‑66 (MQ=255) gCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa > 1:2923855/1‑66 (MQ=255) gCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa > 1:466845/1‑66 (MQ=255) gCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTa > 1:1855344/1‑66 (MQ=255) atatTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATc > 1:1294376/1‑68 (MQ=255) atatTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATc > 1:799927/1‑68 (MQ=255) atatTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATc > 1:2725710/1‑68 (MQ=255) atatTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATc > 1:1581820/1‑68 (MQ=255) atTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaa < 1:2861423/68‑1 (MQ=255) atTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaa < 1:2775502/68‑1 (MQ=255) atTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaa < 1:1614567/68‑1 (MQ=255) atTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaa < 1:2602931/68‑1 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:2127331/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:2001998/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:2212239/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:1834406/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:1049403/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:1442348/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:3053921/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:1086744/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaaa > 1:923515/1‑69 (MQ=255) ttGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCaaa > 1:2005877/1‑68 (MQ=255) gCCCGTCGCCACGGCGAGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACg < 1:2107303/69‑1 (MQ=255) tCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTg < 1:1913662/67‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCat < 1:2506281/69‑2 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1251970/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2653957/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2809510/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:284293/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1554184/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1298016/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:3041363/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:231192/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1257073/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:3073582/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:3433906/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1774824/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2181760/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2135035/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1792196/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:211025/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1829266/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1232496/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2098869/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:497760/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:513597/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2005936/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:864768/69‑1 (MQ=255) gcgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:1993969/69‑1 (MQ=255) gcgGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:198720/69‑1 (MQ=255) gcgGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:617244/69‑1 (MQ=255) gcgGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:194764/69‑1 (MQ=255) cgGATTTCTGTCTTAACCCCATTGGCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2700877/68‑1 (MQ=255) cgGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAg < 1:2386697/68‑1 (MQ=255) tAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGAt < 1:3097547/69‑1 (MQ=255) tAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGAt < 1:3027858/69‑1 (MQ=255) cATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGa < 1:2231346/69‑1 (MQ=255) cATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGa < 1:53828/69‑1 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGTTGCCGATGTGATTATCGaa > 1:3090390/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:2136654/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:443054/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:2097042/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:3042651/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:2932448/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:2926984/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:2921953/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:564360/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:628698/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:676933/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:838947/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:1561135/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:852157/2‑67 (MQ=255) tggCGCTGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGaa > 1:85523/2‑67 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc > 1:2048541/1‑69 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc < 1:684436/69‑1 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc > 1:2103766/1‑69 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc < 1:3176359/69‑1 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc < 1:3369194/69‑1 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc < 1:2741636/69‑1 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc > 1:274587/1‑69 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc < 1:2227334/69‑1 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc > 1:2781023/1‑69 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc > 1:2956848/1‑69 (MQ=255) ggCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAAcc < 1:2220245/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGGTGTGATTATCGAAACCAGCgg < 1:1569175/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:2524721/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:1111886/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:702259/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:2801955/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:2587168/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:2037779/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:1770875/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:2371235/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:1647949/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:3223500/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCgg < 1:423656/69‑1 (MQ=255) tGACGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCCAAACCAGCgg < 1:1049703/69‑1 (MQ=255) | ATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGG > minE/905480‑905606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |