| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 906,051 | C→T | 100% | G26G (GGC→GGT) | ycjR → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 906,051 | 0 | C | T | 93.6% | 58.8 / ‑3.6 | 30 | G26G (GGC→GGT) | ycjR | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (0/28); total (0/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTA > minE/905998‑906088 | gCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTgag < 1:2372932/69‑1 (MQ=255)gCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTga < 1:1376010/68‑1 (MQ=255) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATGGACGGCAAACTGCTGGtca < 1:43684/54‑3 (MQ=255) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:818231/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1018864/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:731753/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:706981/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:637915/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:510696/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:487510/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:454484/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:3368781/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:3104272/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:269007/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:249831/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2448267/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2304468/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2286725/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2174353/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2157578/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:205571/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1895740/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:185226/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1838137/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1793638/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1715942/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1538268/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1248523/54‑3 (MQ=38) atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1087332/54‑3 (MQ=38) tatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2500220/53‑3 (MQ=38) | GCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTA > minE/905998‑906088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |