| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 906,069 | T→C | 100% | I32I (ATT→ATC) | ycjR → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 906,069 | 0 | T | C | 85.6% | 69.4 / 2.6 | 42 | I32I (ATT→ATC) | ycjR | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base C (0/35); total (0/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTA > minE/906035‑906132 | atatCAAAGAGATGGGTTTCGATGGTTTTGAGATGGACGGCAAACTGCTGGtca < 1:43684/54‑3 (MQ=255)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:249831/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:818231/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:731753/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:706981/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:637915/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:510696/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:487510/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:454484/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:3368781/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:3104272/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:269007/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1018864/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2448267/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2304468/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2286725/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2157578/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1538268/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1715942/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1793638/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1838137/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:185226/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1248523/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1087332/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:1895740/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2174353/54‑3 (MQ=38)atatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:205571/54‑3 (MQ=38) tatCAAAGAGATGGGTTTCGATGGTTTTGAGATCGACGGCAAACTGCTGGtca < 1:2500220/53‑3 (MQ=38) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:255387/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:2984994/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:3426852/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:350953/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:2381443/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:441063/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGCAGCAATCAAAGa < 1:149922/68‑1 (MQ=255) gATGGTTTTGAGATCGACGGCAAACTGCTGGTCAACAATCTCGAAGAAGTCAAAGAAGCAATCAAAGa < 1:1985041/68‑1 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTa < 1:1435484/68‑1 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTa < 1:1041213/68‑1 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTa < 1:3324551/68‑1 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTa < 1:3051267/68‑1 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTa < 1:2756098/68‑1 (MQ=255) agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTa < 1:1920486/68‑1 (MQ=255) | ATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTA > minE/906035‑906132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |