| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 83,944 | C→T | 49.5% | L268L (CTG→TTG) | ftsW → | integral membrane protein involved in stabilising FstZ ring during cell division |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 83,944 | 0 | C | T | 49.5% | 2.3 / 98.4 | 107 | L268L (CTG→TTG) | ftsW | integral membrane protein involved in stabilising FstZ ring during cell division |
| Reads supporting (aligned to +/- strand): ref base C (22/32); new base T (42/11); total (64/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTA > minE/83877‑84011 | ccgTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt < 1:1120252/69‑1 (MQ=255)ccgTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt < 1:3183570/69‑1 (MQ=255) ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt < 1:9705/64‑1 (MQ=255) ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt < 1:2757739/64‑1 (MQ=255) ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt > 1:1299951/1‑64 (MQ=255) ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt > 1:1770627/1‑64 (MQ=255) ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt < 1:2423947/64‑1 (MQ=255) ttACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACCCAATCGCt < 1:2473811/64‑1 (MQ=255) aCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCg > 1:3080845/1‑69 (MQ=255) aCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCg > 1:2988890/1‑69 (MQ=255) aCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCg > 1:2913062/1‑69 (MQ=255) aCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCg > 1:3330965/1‑69 (MQ=255) aCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCg > 1:910910/1‑69 (MQ=255) cATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTg < 1:478/69‑1 (MQ=255) cTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCt < 1:3230130/54‑1 (MQ=255) cTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTcg > 1:1535600/1‑69 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:1538443/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:1618889/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:2276695/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:1002597/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:1835293/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:862474/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:797777/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGaa < 1:1958767/69‑1 (MQ=255) ccGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGGCGCGGCGaa < 1:460399/69‑1 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCgcgg > 1:2046222/1‑51 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACtg > 1:3081069/1‑57 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTggg > 1:912152/1‑62 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2449906/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1032817/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1041866/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2863094/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2742598/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2689384/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:888530/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1377736/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:3126020/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:645831/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1621978/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2402882/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:3163902/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1707862/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1976847/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:3184084/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1993843/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2022332/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:213836/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2091244/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:828528/1‑69 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAg > 1:2321157/1‑68 (MQ=255) cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAg > 1:3214470/1‑68 (MQ=255) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:2621106/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:3363617/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:2670980/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:612853/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:1576975/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:2797751/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:308580/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:285133/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:1916765/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:428686/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:1998255/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:1946045/1‑37 (MQ=37) aGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGa > 1:170877/1‑37 (MQ=37) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:393344/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:3028158/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:2970635/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:2172894/1‑68 (MQ=255) tAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACaaa < 1:1304616/69‑1 (MQ=255) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:236951/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:1128627/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:1237706/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:455061/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:201992/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:588438/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:626565/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:2482609/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:2821219/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGATGAACTGTGGGGACAAGGttt < 1:2628487/51‑1 (MQ=255) tCGCTGATGGGGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGa > 1:1587064/1‑68 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:69436/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:2610414/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:1098543/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:114181/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:3099613/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:1348281/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:2131759/1‑69 (MQ=255) gCTGATGGCGTTTTGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:340490/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2397804/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:978898/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:1346005/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:1864786/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2143900/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2436548/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2452635/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3038392/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3119967/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3287423/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3385140/69‑1 (MQ=255) cTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2488172/68‑1 (MQ=255) | CCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTA > minE/83877‑84011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |