| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 913,128 | G→A | 31.8% | S258S (AGC→AGT) | ycjW ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 913,128 | 0 | G | A | 31.8% | 85.2 / 45.6 | 94 | S258S (AGC→AGT) | ycjW | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (47/17); new base A (23/7); total (70/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
CACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTG > minE/913071‑913193 | caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:1313914/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:1873007/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:3338431/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:1608838/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:526963/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:57940/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:1289037/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:299406/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:2634769/1‑67 (MQ=255)caccacCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:258796/1‑67 (MQ=255)caccacAGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAAt > 1:1571484/1‑67 (MQ=255) accGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCAt > 1:2083578/1‑59 (MQ=255) accGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCa > 1:1513530/1‑67 (MQ=255) accGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAgg > 1:2144948/1‑69 (MQ=255) accGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAgg > 1:3071148/1‑69 (MQ=255) accGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAgg > 1:994302/1‑69 (MQ=255) accGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAgg > 1:3243400/1‑69 (MQ=255) gCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCt > 1:982383/1‑68 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:2299572/69‑1 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:2697844/69‑1 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:105674/69‑1 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:313307/69‑1 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:3173068/69‑1 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:1151123/69‑1 (MQ=255) cTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt < 1:610724/69‑1 (MQ=255) tAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGt > 1:980715/1‑68 (MQ=255) tAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTc > 1:3307896/1‑69 (MQ=255) tAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTc < 1:1868210/69‑1 (MQ=255) aCTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTc < 1:2766441/67‑1 (MQ=255) aCTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTc < 1:2888612/67‑1 (MQ=255) ctgcGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTc < 1:1803285/63‑1 (MQ=255) aaTAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAacac < 1:1887910/69‑1 (MQ=255) aaTAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAacac < 1:1824873/69‑1 (MQ=255) gCAATGTTTTTCTCTGCCGCCTCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:3223044/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:458528/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:3326042/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:667197/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:455441/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2872244/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2819403/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:363657/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:904660/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2735041/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:3349445/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:995923/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2564060/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:1160422/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:1794699/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:1846566/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:1971371/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:205288/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2090814/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2193029/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2283437/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2566741/1‑68 (MQ=255) gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc > 1:2395207/1‑68 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTc > 1:787630/1‑53 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc > 1:1444230/1‑68 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc > 1:1747874/1‑68 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc > 1:934858/1‑68 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc > 1:3228163/1‑68 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc > 1:1168473/1‑68 (MQ=255) gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc > 1:2170423/1‑68 (MQ=255) tttttCTCTGCTGCCGCAGGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:2059588/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGcc > 1:409359/1‑63 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:952814/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:993620/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1172037/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:811409/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc < 1:800806/69‑1 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1142495/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1250054/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1044638/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:129831/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:2210100/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1809245/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:2853894/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:2890064/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:2387927/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1887843/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc < 1:26251/69‑1 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:332196/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:1489955/1‑69 (MQ=255) tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc > 1:2605905/1‑69 (MQ=255) ctgctgCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCGGGCAACGGt < 1:434800/68‑1 (MQ=255) ctgctgCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGt < 1:521871/68‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:759275/69‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:703026/69‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:1178557/69‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:1772127/69‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:1961725/69‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:2549699/69‑1 (MQ=255) gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt < 1:2744521/69‑1 (MQ=255) aaTGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTg > 1:1545288/1‑69 (MQ=255) | CACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTG > minE/913071‑913193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |