Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 913,161 C→T 31.8% A247A (GCG→GCA ycjW ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE913,1610CT31.8% 83.9 / 46.8 94A247A (GCG→GCAycjWpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (59/5);  new base T (23/7);  total (82/12)
Fisher's exact test for biased strand distribution p-value = 4.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATA  >  minE/913096‑913226
                                                                 |                                                                 
gCAATGTTTTTCTCTGCCGCCTCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:3223044/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:995923/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2564060/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2395207/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2566741/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2283437/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2193029/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2735041/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2819403/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2090814/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:2872244/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:205288/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:1971371/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:1846566/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:1794699/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:3326042/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:363657/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:3349445/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:455441/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:904660/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:458528/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:1160422/1‑68 (MQ=255)
gCAATGTTTTTCTCTGCCGCCGCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGc                                                                 >  1:667197/1‑68 (MQ=255)
gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc                                                                 >  1:1747874/1‑68 (MQ=255)
gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc                                                                 >  1:2170423/1‑68 (MQ=255)
gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc                                                                 >  1:934858/1‑68 (MQ=255)
gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc                                                                 >  1:1168473/1‑68 (MQ=255)
gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc                                                                 >  1:1444230/1‑68 (MQ=255)
gCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGc                                                                 >  1:3228163/1‑68 (MQ=255)
      tttttCTCTGCTGCCGCAGGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:2059588/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGcc                                                                >  1:409359/1‑63 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:993620/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:2605905/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          <  1:26251/69‑1 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:952814/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:2853894/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:811409/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          <  1:800806/69‑1 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:2890064/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:332196/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1044638/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1142495/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1172037/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:2387927/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:2210100/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1250054/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1887843/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1809245/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:129831/1‑69 (MQ=255)
      tttttCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGc                                                          >  1:1489955/1‑69 (MQ=255)
             ctgctgCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCGGGCAACGGt                                                    <  1:434800/68‑1 (MQ=255)
             ctgctgCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGt                                                    <  1:521871/68‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:1961725/69‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:2549699/69‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:1772127/69‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:703026/69‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:759275/69‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:1178557/69‑1 (MQ=255)
                     gCACGATAAATACTCATTAATTTCAGGCTGTCAGTAGCAAACACTGCCTCAGGCAACGGTGACTGGCtt                                           <  1:2744521/69‑1 (MQ=255)
                             aaTGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTg                                   >  1:1545288/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:66212/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:2783117/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:2982/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:940310/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:2167684/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:870538/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:400632/1‑69 (MQ=255)
                                                 tGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAg               >  1:1290186/1‑69 (MQ=255)
                                                     aGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTc           >  1:3098196/1‑69 (MQ=255)
                                                     aGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTc           >  1:3145461/1‑69 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTtaat                                        >  1:1604515/1‑36 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTtaat                                        >  1:848889/1‑36 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTATTAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:2453200/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:911676/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:936099/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:2537581/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:3336488/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:2917386/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:1985996/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:3273620/1‑68 (MQ=255)
                                                         gCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATg        >  1:2821457/1‑68 (MQ=255)
                                                            aaCACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGttt            >  1:483847/1‑61 (MQ=255)
                                                             acacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGtat   <  1:1218737/69‑1 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:2136/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:1573793/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:1002010/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:280536/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:2105251/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:2616171/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:2089985/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:699913/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:1322007/1‑49 (MQ=255)
                                                              cacCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCtttt                      >  1:2558636/1‑49 (MQ=255)
                                                               acCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGtata  >  1:3349039/1‑68 (MQ=255)
                                                                 |                                                                 
GCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATA  >  minE/913096‑913226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: