| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 915,319 | T→A | 26.4% | P421P (CCT→CCA) | ycjX → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 915,319 | 0 | T | A | 26.4% | 104.8 / 38.6 | 72 | P421P (CCT→CCA) | ycjX | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base T (7/46); new base A (6/13); total (13/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGG > minE/915256‑915380 | tAATCGACTCAGCGATGGAGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCAGGTc > 1:1023472/1‑68 (MQ=255)tAATCGACTCAGCGATGGAGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCAGGTc > 1:39874/1‑68 (MQ=255)tAATCGACTCAGCGATGGAGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCAGGTCa > 1:1233286/1‑69 (MQ=255)tAATCGACTCAGCGATGGAGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCAGGTCa > 1:2399660/1‑69 (MQ=255) gACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGt < 1:2505303/69‑1 (MQ=255) gACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGt < 1:2009501/69‑1 (MQ=255) gACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGt < 1:3262538/69‑1 (MQ=255) gTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGt > 1:2733528/1‑67 (MQ=255) gTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGt > 1:2499804/1‑67 (MQ=255) gTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGt > 1:2918708/1‑67 (MQ=255) gTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGt > 1:3285987/1‑67 (MQ=255) gTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGt > 1:36533/1‑67 (MQ=255) tttATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTg > 1:527330/1‑69 (MQ=255) tATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGa < 1:349613/68‑1 (MQ=255) tATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGa < 1:556013/68‑1 (MQ=255) tATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGa < 1:3154880/68‑1 (MQ=255) tATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGa < 1:960811/68‑1 (MQ=255) tATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGa > 1:2143252/1‑68 (MQ=255) cTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt < 1:358366/69‑1 (MQ=255) cTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt < 1:872505/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:2977994/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:852137/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:275917/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:3039245/69‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:827031/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:2859873/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:458830/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:751816/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:3112420/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:976993/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1364723/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1379353/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1624435/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:2687971/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:186824/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1924692/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1994818/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTCCGCa > 1:800615/1‑69 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTCCGCa > 1:1555790/1‑69 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGGAGGt < 1:962342/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:3348317/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:596902/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:595173/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:342062/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2929116/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2162425/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2880410/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2988937/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTCTGAGGCATTTCGTCCGCAGGt < 1:2687519/68‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATATGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:2983450/69‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:1436313/69‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:2950854/69‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:1432385/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg < 1:3172921/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg < 1:285602/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg < 1:1451132/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:1647191/68‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1384154/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:844214/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1488252/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2730110/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:968703/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2725154/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2326523/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1440726/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2278188/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1660983/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1772923/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:3324949/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1803338/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2070250/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1954836/59‑1 (MQ=255) | TAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGG > minE/915256‑915380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |