Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 915,361 A→G 20.4% A435A (GCA→GCG ycjX → conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE915,3610AG20.4% 102.3 / 18.3 73A435A (GCA→GCGycjXconserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (11/47);  new base G (2/13);  total (13/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGC  >  minE/915294‑915417
                                                                   |                                                        
cTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt                                                         <  1:872505/69‑1 (MQ=255)
cTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt                                                         <  1:358366/69‑1 (MQ=255)
          tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa                                               <  1:852137/69‑1 (MQ=255)
          tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa                                               <  1:275917/69‑1 (MQ=255)
          tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa                                               <  1:3039245/69‑1 (MQ=255)
          tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa                                               <  1:2977994/69‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:3112420/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:186824/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:1924692/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:1994818/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:2687971/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:2859873/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:1364723/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:458830/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:751816/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:827031/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:976993/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:1379353/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc                                                  <  1:1624435/66‑1 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTCCGCa                                               >  1:1555790/1‑69 (MQ=255)
          tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTCCGCa                                               >  1:800615/1‑69 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGGAGGt                                            <  1:962342/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:342062/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:2929116/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:2880410/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:2988937/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:3348317/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:595173/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:596902/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt                                            <  1:2162425/68‑1 (MQ=255)
              gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTCTGAGGCATTTCGTCCGCAGGt                                            <  1:2687519/68‑1 (MQ=255)
                 cGTTTGCCTGGTCAGGCGTTCTGGGATATGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg                                        <  1:2983450/69‑1 (MQ=255)
                 cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg                                        <  1:2950854/69‑1 (MQ=255)
                 cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg                                        <  1:1436313/69‑1 (MQ=255)
                 cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg                                        <  1:1432385/69‑1 (MQ=255)
                  gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg                                       <  1:1451132/69‑1 (MQ=255)
                  gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg                                       <  1:3172921/69‑1 (MQ=255)
                  gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg                                       <  1:285602/69‑1 (MQ=255)
                  gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg                                        <  1:1647191/68‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:968703/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1384154/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:844214/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1488252/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:3324949/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1803338/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1440726/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:2730110/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1954836/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1772923/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:2070250/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:2278188/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:2326523/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:2725154/59‑1 (MQ=255)
                   tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                                <  1:1660983/59‑1 (MQ=255)
                                gCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAAcc                          <  1:2214443/68‑1 (MQ=255)
                                gCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAAcc                          <  1:62471/68‑1 (MQ=255)
                                gCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAAcc                          <  1:2322572/68‑1 (MQ=255)
                                    tCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTAc                     <  1:2633819/69‑1 (MQ=255)
                                    tCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTAc                     <  1:2425656/69‑1 (MQ=255)
                                     cTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTAc                     <  1:1196579/68‑1 (MQ=255)
                                          aTAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCata               >  1:2096249/1‑69 (MQ=255)
                                          aTAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCata               >  1:1991261/1‑69 (MQ=255)
                                             aGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCTGGTgatg                                        <  1:2602853/41‑1 (MQ=255)
                                                aaGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTc         <  1:1454651/69‑1 (MQ=255)
                                                       ccAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGAtgc  >  1:2910201/1‑69 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:3226744/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:2393964/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:34375/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:2596559/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:3363576/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:957316/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg           >  1:1833134/1‑54 (MQ=255)
                                                             tGAGGCATTTCGTCCGCAGGTGATGGAAGTCGACAAACCACTACCGCATATTCg           >  1:837430/1‑54 (MQ=255)
                                                                   |                                                        
CTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGC  >  minE/915294‑915417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: