Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 915,361 | A→G | 20.4% | A435A (GCA→GCG) | ycjX → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 915,361 | 0 | A | G | 20.4% | 102.3 / 18.3 | 73 | A435A (GCA→GCG) | ycjX | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
Reads supporting (aligned to +/- strand): ref base A (11/47); new base G (2/13); total (13/60) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
CTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGC > minE/915294‑915417 | cTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt < 1:872505/69‑1 (MQ=255) cTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt < 1:358366/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:852137/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:275917/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:3039245/69‑1 (MQ=255) tCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCa < 1:2977994/69‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:3112420/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:186824/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1924692/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1994818/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:2687971/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:2859873/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1364723/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:458830/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:751816/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:827031/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:976993/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1379353/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTcc < 1:1624435/66‑1 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTCCGCa > 1:1555790/1‑69 (MQ=255) tCCCGCACGTTTGCCAGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCGTTTCGTCCGCa > 1:800615/1‑69 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGGAGGt < 1:962342/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:342062/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2929116/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2880410/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2988937/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:3348317/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:595173/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:596902/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGt < 1:2162425/68‑1 (MQ=255) gCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTCTGAGGCATTTCGTCCGCAGGt < 1:2687519/68‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATATGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:2983450/69‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:2950854/69‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:1436313/69‑1 (MQ=255) cGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:1432385/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg < 1:1451132/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg < 1:3172921/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatgg < 1:285602/69‑1 (MQ=255) gTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatg < 1:1647191/68‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:968703/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1384154/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:844214/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1488252/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:3324949/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1803338/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1440726/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2730110/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1954836/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1772923/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2070250/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2278188/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2326523/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:2725154/59‑1 (MQ=255) tttGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc < 1:1660983/59‑1 (MQ=255) gCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAAcc < 1:2214443/68‑1 (MQ=255) gCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAAcc < 1:62471/68‑1 (MQ=255) gCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAAcc < 1:2322572/68‑1 (MQ=255) tCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTAc < 1:2633819/69‑1 (MQ=255) tCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTAc < 1:2425656/69‑1 (MQ=255) cTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTAc < 1:1196579/68‑1 (MQ=255) aTAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCata > 1:2096249/1‑69 (MQ=255) aTAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCata > 1:1991261/1‑69 (MQ=255) aGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCTGGTgatg < 1:2602853/41‑1 (MQ=255) aaGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTc < 1:1454651/69‑1 (MQ=255) ccAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGAtgc > 1:2910201/1‑69 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:3226744/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:2393964/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:34375/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:2596559/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:3363576/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:957316/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCg > 1:1833134/1‑54 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGAAGTCGACAAACCACTACCGCATATTCg > 1:837430/1‑54 (MQ=255) | CTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGC > minE/915294‑915417 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |