| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 915,579 | C→T | 30.8% | D43D (GAC→GAT) | ycjF → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 915,579 | 0 | C | T | 30.8% | 66.0 / 31.4 | 71 | D43D (GAC→GAT) | ycjF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (25/24); new base T (4/18); total (29/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCC > minE/915517‑915646 | ttCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAgcg < 1:210617/69‑1 (MQ=255)ttCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAgcg < 1:1177808/69‑1 (MQ=255)ttCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAgcg < 1:1228726/69‑1 (MQ=255)ttCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAgcg < 1:3363662/69‑1 (MQ=255) cgcAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGga > 1:1659562/1‑67 (MQ=255) cgcAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGga > 1:188140/1‑67 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:3224182/69‑1 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:2381961/69‑1 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:169710/69‑1 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:2724656/69‑1 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:1556509/69‑1 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:422238/69‑1 (MQ=255) cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGgaagaa < 1:467506/69‑1 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:3029474/1‑68 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:3009578/1‑68 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:2276362/1‑68 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:2592850/1‑68 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:2542177/1‑68 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:1960599/1‑68 (MQ=255) gACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAgg > 1:2331430/1‑68 (MQ=255) cTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGACACGCTCGACGAAGCGCAGGAAGAAGAGGGGCa > 1:3210374/1‑69 (MQ=255) aCGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGt > 1:144512/1‑67 (MQ=255) aCGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGt > 1:45633/1‑67 (MQ=255) gAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGa < 1:3240454/68‑1 (MQ=255) gAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGa < 1:1841183/68‑1 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:3044757/1‑69 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:3369714/1‑69 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:472637/1‑69 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:713080/1‑69 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:2435398/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:2221946/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:977760/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:1597176/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:1125791/69‑1 (MQ=255) cAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGaagaag < 1:1905728/49‑1 (MQ=255) cAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGaagaag < 1:2495635/49‑1 (MQ=255) cAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGaagaag < 1:2967937/49‑1 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAgg > 1:1111680/1‑42 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:1046424/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:37611/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:1444269/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:3105065/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:2980140/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:649640/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:2709471/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:260296/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:172796/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:945360/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:1790034/1‑69 (MQ=255) aGGCGCAAAATTTTGCCC‑‑‑CCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATgg > 1:1662482/1‑69 (MQ=255) gcgcAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCACGAAGAAGAGGGGCAAGTCGAAGCGGTAATg < 1:1793776/69‑1 (MQ=255) ccACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa < 1:2567360/69‑1 (MQ=255) ccACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa < 1:2441553/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGTTGATGGACGCAGCGTTACGTCCGAAAc < 1:815063/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:964576/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1328745/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1589825/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:3426919/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1480938/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1504681/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1507664/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1913508/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:2993510/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1684446/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:266895/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1758869/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1011695/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:2237889/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGT‑ATGGACGCAGCGTTACGTCCGAAAc < 1:1559644/68‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGATGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1516707/69‑1 (MQ=255) gCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1133543/68‑1 (MQ=255) cgaAGCTCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGcc < 1:2770059/68‑1 (MQ=255) cgaAGCGCATGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGGTACGTCCGAAACGCAGcc < 1:3217680/68‑1 (MQ=255) cgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGcc < 1:676010/68‑1 (MQ=255) | TTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCC > minE/915517‑915646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |