| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 915,606 | C→T | 28.4% | V52V (GTC→GTT) | ycjF → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 915,606 | 0 | C | T | 28.4% | 90.0 / 34.0 | 84 | V52V (GTC→GTT) | ycjF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (30/30); new base T (4/20); total (34/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCT > minE/915541‑915674 | gAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGa < 1:1841183/68‑1 (MQ=255)gAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGa < 1:3240454/68‑1 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:713080/1‑69 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:472637/1‑69 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:3044757/1‑69 (MQ=255) aaTCAGGCGCAAAATTTTGCCCCAGCCACGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCg > 1:3369714/1‑69 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:977760/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:1597176/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:2221946/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:2435398/69‑1 (MQ=255) tCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGt < 1:1125791/69‑1 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:3105065/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:37611/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:2980140/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:2709471/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:260296/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:649640/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:945360/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:1790034/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:172796/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:1046424/1‑69 (MQ=255) aGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa > 1:1444269/1‑69 (MQ=255) aGGCGCAAAATTTTGCCC‑‑‑CCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATgg > 1:1662482/1‑69 (MQ=255) gcgcAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCACGAAGAAGAGGGGCAAGTCGAAGCGGTAATg < 1:1793776/69‑1 (MQ=255) ccACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa < 1:2567360/69‑1 (MQ=255) ccACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa < 1:2441553/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGTTGATGGACGCAGCGTTACGTCCGAAAc < 1:815063/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1011695/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:3426919/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1328745/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:2993510/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1480938/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:266895/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1504681/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1507664/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:964576/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:2237889/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1684446/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1913508/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1589825/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1758869/69‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGT‑ATGGACGCAGCGTTACGTCCGAAAc < 1:1559644/68‑1 (MQ=255) cGCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGATGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1516707/69‑1 (MQ=255) gCTCGATGAAGCGCAGGAAGAAGAGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:1133543/68‑1 (MQ=255) cgaAGCTCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGcc < 1:2770059/68‑1 (MQ=255) cgaAGCGCATGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGGTACGTCCGAAACGCAGcc < 1:3217680/68‑1 (MQ=255) cgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGcc < 1:676010/68‑1 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGCCGCAGCGTTACGTCCGAAACg > 1:2165309/1‑56 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGgcg > 1:598379/1‑68 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGgcg > 1:72454/1‑68 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGgcg > 1:168752/1‑68 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGgcg > 1:1468547/1‑68 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGgcg > 1:2705674/1‑68 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGgcg > 1:1577676/1‑68 (MQ=255) cAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTT‑CGTCCGAAACGCAGCCTGTGgcgc > 1:2021488/1‑68 (MQ=255) aagaGGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:645265/48‑1 (MQ=255) agagGGGCAAGTTGAAGCGGTGATGGACGCAGCGTTACGTCCGAAAc < 1:3377214/47‑1 (MQ=255) agagGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:3080764/69‑1 (MQ=255) agagGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:2069287/69‑1 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTgg > 1:3430697/1‑53 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCa > 1:1187723/1‑57 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:2074549/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:2370837/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:1674879/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:2311082/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:3000369/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:2619855/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGAt > 1:370872/1‑67 (MQ=255) ggCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCC‑GTGGCGCAAAATGGTGATggg > 1:2088961/1‑69 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:147750/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:109367/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:1131305/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:926405/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:1145204/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:373633/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:3380164/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:1196360/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:3220863/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:3154227/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:3079792/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:2287173/63‑1 (MQ=255) gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGt < 1:2245020/63‑1 (MQ=255) aaGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAg < 1:2580212/69‑1 (MQ=255) aaGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAg < 1:1667582/69‑1 (MQ=255) cGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGggct > 1:953434/1‑69 (MQ=255) | GAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCT > minE/915541‑915674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |