| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 917,229 | C→T | 100% | R190R (CGC→CGT) | tyrR → | DNA‑binding transcriptional dual regulator, tyrosine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 917,229 | 0 | C | T | 85.9% | 18.8 / ‑2.7 | 14 | R190R (CGC→CGT) | tyrR | DNA‑binding transcriptional dual regulator, tyrosine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (4/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGTTGCGATCAACGATTCGTATGGGCCGCCAGTTGCAAAATGTCG > minE/917163‑917245 | tGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGTTGCGATCAACGATTCGTATGGGCCGCCa > 1:1391090/1‑69 (MQ=255)tGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGTTGCGATCAACGATTCGTATGGGCCGCCa > 1:1589164/1‑69 (MQ=255)tGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGGCGTCa > 1:2439179/1‑69 (MQ=255)tGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCa > 1:2787567/1‑69 (MQ=255)tGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCa > 1:3336219/1‑69 (MQ=255)tGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCa > 1:612908/1‑69 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:1052739/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:1377107/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:1393799/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:2586921/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:2755444/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:3138061/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:3412636/69‑1 (MQ=255) acGTCCTGACCGGTGCGGTGGTGATGCTGCGATCAACGATTCGTATGGGCCGTCAGTTGCAAAATGTcg < 1:977749/69‑1 (MQ=255) | TGAAAATGATCAACACGTCCTGACCGGTGCGGTGGTGATGTTGCGATCAACGATTCGTATGGGCCGCCAGTTGCAAAATGTCG > minE/917163‑917245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |