| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 920,527 | G→A | 100% | T24T (ACC→ACT) | mpaA ← | murein peptide amidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 920,527 | 0 | G | A | 77.4% | 23.6 / 2.7 | 22 | T24T (ACC→ACT) | mpaA | murein peptide amidase A |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (0/17); total (2/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAG > minE/920472‑920567 | aaCGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTAc < 1:1141508/61‑1 (MQ=255) aaTGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTg > 1:2716793/1‑69 (MQ=255) ccGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAg > 1:1611318/1‑68 (MQ=255) ccGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAg < 1:1771384/68‑1 (MQ=255) ggCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTAt < 1:3358567/68‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:197298/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:702227/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:64702/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:505566/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:3351141/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:3056396/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:2518584/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:2357090/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1956239/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1952228/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1875204/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1567287/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1535876/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1506765/67‑1 (MQ=255) aaTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1156652/67‑1 (MQ=255) aTGCGCCGCGTTCGGCGCGTGGGCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:1337319/66‑1 (MQ=255) gggCGAGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAg < 1:2580578/46‑1 (MQ=255) | AACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAG > minE/920472‑920567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |