BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	921,380	G→A	35.8%	T142T (ACC→ACT)	ycjY ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	921,380	0	G	A	35.8%	120.4 / 101.2	167	T142T (ACC→ACT)	ycjY	predicted hydrolase
Reads supporting (aligned to +/- strand): ref base G (54/53); new base A (28/32); total (82/85)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

Only 100 of 184 total aligned reads displayed.

AATAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGCACCGGCACAAA  >  minE/921317‑921447

aagaGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTAcc                                                                >  1:1466498/4‑69 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:3176453/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:1714263/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:1821870/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:2986839/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:547494/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:2782318/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:866620/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    <  1:2573439/65‑1 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:228666/1‑65 (MQ=255)

aaTAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACgg                                                                    >  1:1036640/1‑65 (MQ=255)

   aGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGAt                                                             >  1:3248832/1‑69 (MQ=255)

         cacaTTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       >  1:421040/1‑69 (MQ=255)

            aTTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTAcc                                                                <  1:617573/57‑1 (MQ=255)

             ttGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATg                                                   >  1:1319970/1‑69 (MQ=255)

             ttGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATg                                                   >  1:110195/1‑69 (MQ=255)

             ttGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATg                                                   >  1:3227156/1‑69 (MQ=255)

              tGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATgc                                                  >  1:3020744/1‑69 (MQ=255)

              tGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATgc                                                  >  1:1916342/1‑69 (MQ=255)

                     ccAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGAt                                            <  1:3030200/68‑1 (MQ=255)

                     ccAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGAt                                            <  1:149189/68‑1 (MQ=255)

                        aCCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTg                                        <  1:2903020/69‑1 (MQ=255)

                        aCCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTg                                        <  1:626248/69‑1 (MQ=255)

                        aCCATTACGGAACATCGAACCAATATTGACCGCACTAACAGTACCGATGGCGTTGATGCGGCGATCCt                                         >  1:2933779/1‑68 (MQ=38)

                        aCCATTACGGAACATCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCt                                         >  1:2967408/1‑68 (MQ=255)

                        aCCATTACGGAACATCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTg                                        >  1:1810022/1‑69 (MQ=255)

                        aCCATTACGGAACATCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTg                                        >  1:730653/1‑69 (MQ=255)

                        aCCATTACGGAACATCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTg                                        >  1:478621/1‑69 (MQ=255)

                        aCCATTACGGAACATCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTg                                        >  1:3029467/1‑69 (MQ=255)

                         ccATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       <  1:1752102/53‑1 (MQ=255)

                         ccATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       <  1:2526713/53‑1 (MQ=255)

                         ccATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       <  1:590413/53‑1 (MQ=255)

                         ccATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       <  1:782849/53‑1 (MQ=255)

                         ccATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       <  1:3316354/53‑1 (MQ=255)

                         ccATTACGGAATATCGAACAAATATTGACCGCACTAACGGTACCGATGGCCtt                                                       <  1:1141491/53‑1 (MQ=255)

                              aCGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgc                                  <  1:1414927/69‑1 (MQ=255)

                              aCGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgc                                  <  1:908807/69‑1 (MQ=255)

                              aCGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgc                                  <  1:716812/69‑1 (MQ=255)

                              aCGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGAT‑‑‑GCGATCCTGAATTgcagc                               <  1:2906478/69‑1 (MQ=255)

                                ggAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATgc                                                  <  1:348264/51‑1 (MQ=255)

                                 gAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagc                               <  1:1010374/69‑1 (MQ=255)

                                 gAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagc                               <  1:381053/69‑1 (MQ=255)

                                  aacatCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagca                              >  1:2337072/4‑69 (MQ=255)

                                  aacatCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagca                              >  1:1535457/4‑69 (MQ=255)

                                  aacatCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagca                              >  1:161869/4‑69 (MQ=255)

                                  aacatCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagca                              >  1:1792273/4‑69 (MQ=255)

                                  aacatCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagca                              >  1:2759471/4‑69 (MQ=255)

                                  aacatCGAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagca                              >  1:2582826/4‑69 (MQ=255)

                                  aaTATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcagc                               <  1:3355029/68‑1 (MQ=255)

                                   atatCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATgcg                                                 >  1:2128854/1‑49 (MQ=255)

                                   atatCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCAt                             >  1:286779/1‑69 (MQ=255)

                                   atatCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCAt                             >  1:368720/1‑69 (MQ=255)

                                   atatCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCAt                             >  1:2244471/1‑69 (MQ=255)

                                   atatCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCAt                             >  1:2715962/1‑69 (MQ=255)

                                   atatCGAAACAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCAt                             >  1:2621431/1‑69 (MQ=255)

                                      tCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTgg                          >  1:3243019/1‑69 (MQ=255)

                                      tCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTgg                          >  1:1201927/1‑69 (MQ=255)

                                       cGAACCAATATTGACCGCACTAACGGTACCGATGTCCTTGATGCGGCGATCCTGAATTGCAGCATTGGc                         >  1:1781241/1‑69 (MQ=255)

                                       cGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGc                         >  1:2147308/1‑69 (MQ=255)

                                       cGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGc                         >  1:1077734/1‑69 (MQ=255)

                                       cGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGc                         >  1:3436410/1‑69 (MQ=255)

                                       cGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGc                         >  1:149825/1‑69 (MQ=255)

                                       cGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGc                         >  1:1112153/1‑69 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCTATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:2054663/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:886705/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:3408622/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:3183193/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:987950/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:1239585/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:3140888/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:3122589/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:135308/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:3039724/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:1432734/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:1666920/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:2035566/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:2224553/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:2246796/69‑1 (MQ=255)

                                        gAACCAATATTGACCGCACTAACAGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCt                        <  1:2426045/69‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTg                       <  1:1060897/68‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTg                       <  1:1929478/68‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTg                       <  1:2360278/68‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTg                       <  1:3090974/68‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGt                      <  1:2433818/69‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGt                      <  1:385501/69‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGt                      <  1:2615738/69‑1 (MQ=255)

                                          aCCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGt                      <  1:1407757/69‑1 (MQ=255)

                                           ccAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGta                     <  1:510848/69‑1 (MQ=255)

                                                 ttGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcag                                >  1:412231/1‑52 (MQ=255)

                                                 ttGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTgcag                                >  1:2664008/1‑52 (MQ=255)

                                                   gACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTg              <  1:1589505/68‑1 (MQ=255)

                                                   gACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGc             >  1:2212647/1‑69 (MQ=255)

                                                   gACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGc             >  1:1522150/1‑69 (MQ=255)

                                                   gACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGc             >  1:2854431/1‑69 (MQ=255)

                                                   gACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCAGc             >  1:2407490/1‑69 (MQ=255)

                                                        cACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTg              >  1:3324322/1‑63 (MQ=255)

                                                        cACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTg              >  1:3152987/1‑63 (MQ=255)

                                                            aaCGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGCACCGGcaca    >  1:3335343/1‑69 (MQ=255)

                                                               ggTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGCACCGGCACaaa  <  1:1000771/68‑1 (MQ=255)

                                                               ggTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGCACCGGCACaaa  <  1:3158671/68‑1 (MQ=255)

AATAGATTTCACATTGTTTTCCCAACCATTACGGAATATCGAACCAATATTGACCGCACTAACGGTACCGATGGCCTTGATGCGGCGATCCTGAATTGCAGCATTGGCTGTATATCCTGCACCGGCACAAA  >  minE/921317‑921447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`