| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 923,755 | G→A | 34.5% | G197S (GGT→AGT) | mppA → | murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 923,755 | 0 | G | A | 34.5% | 59.7 / 44.1 | 81 | G197S (GGT→AGT) | mppA | murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (16/37); new base A (20/8); total (36/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01 | |||||||||||
GCCGTGGTTTGTGAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTA > minE/923688‑923809 | gCCGTGGTTTGTGAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCg > 1:169805/1‑68 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:2975304/1‑69 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:1275425/1‑69 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:414254/1‑69 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:2395983/1‑69 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:724243/1‑69 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:2757523/1‑69 (MQ=255) tgtgAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTg > 1:1631709/1‑69 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:2202344/65‑1 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:2691679/65‑1 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:3417280/65‑1 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:3159085/65‑1 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:1804708/65‑1 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:2413331/65‑1 (MQ=255) aaTTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:336762/65‑1 (MQ=255) aaTTTAACCCCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTg < 1:3119369/65‑1 (MQ=255) tttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaa > 1:2713360/1‑69 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTgg > 1:2070566/1‑63 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaaa > 1:2190668/1‑69 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaaa > 1:1570688/1‑69 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaaa > 1:249731/1‑69 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaaa > 1:1815165/1‑69 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaaa > 1:947265/1‑69 (MQ=255) ttAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGaaa > 1:1292667/1‑69 (MQ=255) tttGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTg < 1:1925555/69‑1 (MQ=255) tGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGAt > 1:2615825/1‑69 (MQ=255) ccTTCTTCCCGGTGCAAAAAGGCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATc < 1:1861062/68‑1 (MQ=255) ccTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATc < 1:1580740/68‑1 (MQ=255) ccTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATc < 1:1512129/68‑1 (MQ=255) ttcttcCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:2340820/69‑1 (MQ=255) ttcttcCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:2486402/69‑1 (MQ=255) ttcttcCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:157138/69‑1 (MQ=255) ttcttcCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:3094948/69‑1 (MQ=255) ttcttcCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:3055490/69‑1 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGAc > 1:2623293/1‑40 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:3134903/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:2745618/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:2805665/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:3032285/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:2166842/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:3310546/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:2359369/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:2296435/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:2241590/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:364083/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:408854/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:45974/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:1681731/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:1630464/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:868058/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:1422249/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:1075236/1‑53 (MQ=255) cGGTGCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:1007577/1‑53 (MQ=255) cGGTCCAAAAAGCCAACGTAGAAAGCAGTAAAGAGTGGACGAAACCCGGAAAt > 1:1557602/1‑53 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:3374037/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:99436/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:3005833/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:1520255/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:1555740/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:2118961/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:2399991/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:2723539/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:2767507/69‑1 (MQ=255) gTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCt < 1:2805731/69‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:2447974/57‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:2289614/57‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:3420883/57‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:3422474/57‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:349924/57‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:1938417/57‑1 (MQ=255) cAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:872126/57‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:948534/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:1124009/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:919997/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:1015291/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:1883956/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:2146159/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:2512600/69‑1 (MQ=255) ccAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:2810477/69‑1 (MQ=255) cAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTa < 1:1983289/68‑1 (MQ=255) gTAGAAAGCGGTAAAGAGTGGACGAAACCCGTAAATCTGATCGGc < 1:918443/45‑1 (MQ=255) aaaGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGc < 1:825151/41‑1 (MQ=255) | GCCGTGGTTTGTGAATTTAACCGCTAACTTTGCCTTCTTCCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTCTTA > minE/923688‑923809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |