| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 924,732 | T→C | 14.5% | N522N (AAT→AAC) | mppA → | murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 924,732 | 0 | T | C | 14.5% | 274.0 / 27.3 | 157 | N522N (AAT→AAC) | mppA | murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (78/56); new base C (5/18); total (83/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 186 total aligned reads displayed.
GCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTT > minE/924664‑924799 | gCACCAATTTATCAATATACCAATGGCCGATTAATCAAGCCGTGGCTGAATGGTTATCCCATTaataat > 1:2017893/1‑69 (MQ=255)gCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTaataat > 1:1142043/1‑69 (MQ=255)gCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTaataat > 1:265978/1‑69 (MQ=255)gCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTaataat > 1:3310864/1‑69 (MQ=255)gCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTaataat > 1:571701/1‑69 (MQ=255)gCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTaataat > 1:2272665/1‑69 (MQ=255) tATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGAt > 1:198756/1‑69 (MQ=255) tATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGAt > 1:48324/1‑69 (MQ=255) tATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGAt > 1:2143071/1‑69 (MQ=255) tCAATATACCAAT‑‑ACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGAtgtg < 1:1068490/68‑1 (MQ=255) tCAATATACCAAT‑‑ACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATACTGAAGAtgtg < 1:332358/68‑1 (MQ=255) tataCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg < 1:653502/69‑1 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATTATCCTGAAGATGTGGCg > 1:189350/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:647445/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:412353/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:2286463/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:3119280/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:2160355/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:871976/1‑67 (MQ=255) taCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCg > 1:2204431/1‑67 (MQ=255) cAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAg > 1:2472798/1‑69 (MQ=255) cAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAg > 1:2811173/1‑69 (MQ=255) cAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAg > 1:3336355/1‑69 (MQ=255) cAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAg > 1:1001333/1‑69 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTgg > 1:1517055/1‑57 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:47502/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:2171692/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:2003243/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:2203712/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:2303248/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:2346876/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:1000316/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:1575720/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:1458597/1‑68 (MQ=255) gACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCgg > 1:2635680/1‑68 (MQ=255) cGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:360204/69‑1 (MQ=255) cGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:2111579/69‑1 (MQ=255) cGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:1360077/69‑1 (MQ=255) cGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:2602002/69‑1 (MQ=255) cGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:3004266/69‑1 (MQ=255) cGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:222380/69‑1 (MQ=255) gATTAATCAAGCCGTGTCTGAAAGGTTATCCCATTAATAACCCTGAAGATGTGGCGTACAGTCGGACt < 1:3382010/68‑1 (MQ=255) ttAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:1899687/69‑1 (MQ=255) ttAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:2743394/69‑1 (MQ=255) ttAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:418298/69‑1 (MQ=255) ttAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:812710/69‑1 (MQ=255) ttAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:1619963/69‑1 (MQ=255) ttAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:1256048/69‑1 (MQ=255) ttAATCAAGCCGTGGCTCAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:2055952/69‑1 (MQ=255) atAATCAAGCCGTGGCTGAAAGGTTATCCCATTAAAAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:1118905/68‑1 (MQ=255) tAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg > 1:1341405/1‑68 (MQ=255) tAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg > 1:1173233/1‑68 (MQ=255) tAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg > 1:1404841/1‑68 (MQ=255) tAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg > 1:3256629/1‑68 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:2058098/1‑69 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:767856/1‑69 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:1269396/1‑69 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:3175846/1‑69 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:2966561/1‑69 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:1828476/1‑69 (MQ=255) cAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTatat > 1:2949512/1‑69 (MQ=255) tGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAg < 1:3095078/69‑1 (MQ=255) tGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAg < 1:2446190/69‑1 (MQ=255) ggCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:1645460/56‑1 (MQ=255) gCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCa < 1:127496/69‑1 (MQ=255) gCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCa < 1:2461359/69‑1 (MQ=255) gCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCa < 1:2501086/69‑1 (MQ=255) gCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCa < 1:3090607/69‑1 (MQ=255) gAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATg < 1:257835/52‑1 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt < 1:1858392/69‑1 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt < 1:951189/69‑1 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt < 1:1749592/69‑1 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt > 1:2353063/1‑69 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt > 1:2625928/1‑69 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt > 1:2979268/1‑69 (MQ=255) ggTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt < 1:2863402/69‑1 (MQ=255) gTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgtg > 1:2860663/1‑69 (MQ=255) gTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgtg > 1:2928168/1‑69 (MQ=255) gTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATtgatgt < 1:515281/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:3024960/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:2393906/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:934504/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:1255041/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:2701038/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:670431/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:1668039/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:1684928/68‑1 (MQ=255) ccATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATggg < 1:2856910/68‑1 (MQ=255) taataaTCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTg > 1:1455233/1‑69 (MQ=255) taataaTCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTg > 1:1369688/1‑69 (MQ=255) taataaTCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTg > 1:1761316/1‑69 (MQ=255) aataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:2878928/69‑1 (MQ=255) aataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:1594054/69‑1 (MQ=255) aataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:2902755/69‑1 (MQ=255) aataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:3408476/69‑1 (MQ=255) aataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:702243/69‑1 (MQ=255) aataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:2837201/69‑1 (MQ=255) ataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:1185796/68‑1 (MQ=255) ataacCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTAATGTGATGGGAACTgg < 1:1883030/68‑1 (MQ=255) atCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGtt < 1:1500109/69‑1 (MQ=255) | GCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTT > minE/924664‑924799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |