| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 927,449 | C→T | 36.8% | R82H (CGC→CAC) | narZ ← | nitrate reductase 2 (NRZ), alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 927,449 | 0 | C | T | 36.8% | 27.4 / 24.7 | 46 | R82H (CGC→CAC) | narZ | nitrate reductase 2 (NRZ), alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (16/13); new base T (9/8); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01 | |||||||||||
GTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCA > minE/927382‑927516 | gTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCg < 1:2576449/69‑1 (MQ=255) tAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCg < 1:1994553/53‑1 (MQ=255) aaCTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTTGTCgg > 1:474636/1‑69 (MQ=255) aaCTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTc > 1:1702398/1‑67 (MQ=255) aaCTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCgg > 1:2508594/1‑69 (MQ=255) aaCTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCgg > 1:275540/1‑69 (MQ=255) aCTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGt > 1:3196737/1‑69 (MQ=255) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGTGTGAGTGCGCGGGTAGTCGGTCt < 1:3076884/69‑1 (MQ=25) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:1083143/69‑1 (MQ=25) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:1032255/69‑1 (MQ=25) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:2928509/69‑1 (MQ=25) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:2203989/69‑1 (MQ=25) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:649833/69‑1 (MQ=25) ttGCGCCACGCGGGCAGCCGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:110838/69‑1 (MQ=25) cctcgcgGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:1564724/66‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:3358463/69‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:3385800/69‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:3435093/69‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:2802396/69‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:80634/69‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:2671696/69‑1 (MQ=255) ccACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:2405931/69‑1 (MQ=255) ccGCGAGGTTCGTGATTTGGCAGGTCAGGGTGAGTGCGCGGGTAGTCGGTCt < 1:3358355/52‑1 (MQ=255) gcgAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCAc < 1:541029/69‑1 (MQ=255) cATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCAc < 1:89129/61‑1 (MQ=255) aGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTgg < 1:1905219/36‑1 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCa > 1:2545392/1‑42 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:2539939/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:2435118/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:2327604/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:2822957/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:783958/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:3039055/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:1558/1‑69 (MQ=255) gTCAGGGTGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:353498/1‑69 (MQ=255) tCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTaa > 1:1769446/1‑61 (MQ=255) tCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAtt > 1:1563295/1‑69 (MQ=255) tCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAtt > 1:1391767/1‑69 (MQ=255) tCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAtt > 1:931171/1‑69 (MQ=255) tCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAt > 1:767716/1‑68 (MQ=255) cAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAttt > 1:1650189/1‑69 (MQ=255) cAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAttt > 1:1793217/1‑69 (MQ=255) cAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAttt > 1:645125/1‑69 (MQ=255) cAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAttt > 1:2263206/1‑69 (MQ=255) cAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGAttt > 1:2923156/1‑69 (MQ=255) gCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCa > 1:1371616/1‑69 (MQ=255) | GTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGGTCAGGGCGAGTGCGCGGGTAGTCGGTCTGTTGGATTTCCCAGGTCACCAGACCATTTTTAACGTAGATTTTCCA > minE/927382‑927516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |