| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 931,721 | A→G | 31.5% | N165N (AAT→AAC) | yddG ← | predicted methyl viologen efflux pump |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 931,721 | 0 | A | G | 31.5% | 63.9 / 37.2 | 76 | N165N (AAT→AAC) | yddG | predicted methyl viologen efflux pump |
| Reads supporting (aligned to +/- strand): ref base A (24/28); new base G (15/9); total (39/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAC > minE/931662‑931787 | gTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCcg < 1:2711859/69‑1 (MQ=255) aGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtatt < 1:2853104/69‑1 (MQ=255) aaaCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtattta < 1:1834078/68‑1 (MQ=255) cctcaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2100134/66‑1 (MQ=255) ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:397379/69‑1 (MQ=255) ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2205873/69‑1 (MQ=255) ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:1815197/69‑1 (MQ=255) ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2810786/69‑1 (MQ=255) ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2806860/69‑1 (MQ=255) ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2619338/69‑1 (MQ=255) cacaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:534170/68‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:845488/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2091070/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2032990/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:198512/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2559399/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2636689/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:3076420/69‑1 (MQ=255) acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:1326098/69‑1 (MQ=255) acaGACTAGCTCCCGTTAGCAGGAGAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2158599/69‑1 (MQ=255) ttGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGc < 1:376657/69‑1 (MQ=255) ttGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGc < 1:2117419/69‑1 (MQ=255) gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCt < 1:2401520/69‑1 (MQ=255) gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCt < 1:833516/69‑1 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAg > 1:3422534/1‑65 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:2458729/1‑69 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:3136359/1‑69 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:3287022/1‑69 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:106817/1‑69 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:1644469/1‑69 (MQ=255) cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:1762503/1‑69 (MQ=255) cAAAAACGGTAATTCCATTAAATCAGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:1667716/1‑69 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTATGCTGCCCAGATa > 1:2793897/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1895249/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:470702/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:3424525/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:614558/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:3146589/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:8306/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1480235/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2845365/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1647497/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2199774/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1821838/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2560653/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1779443/1‑67 (MQ=255) aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2409733/1‑67 (MQ=255) aaaCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg < 1:2654154/69‑1 (MQ=255) aaaCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg < 1:2502361/69‑1 (MQ=255) aaCTGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg < 1:1778174/68‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:325339/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:458434/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1207131/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1209938/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:2171030/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1293095/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:33881/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1580137/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:687422/69‑1 (MQ=255) ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1485809/69‑1 (MQ=255) tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGaa > 1:3377981/1‑69 (MQ=255) tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGaa > 1:2689989/1‑69 (MQ=255) tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGaa > 1:1421991/1‑69 (MQ=255) tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGa > 1:524349/1‑68 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1070237/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1120555/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:661163/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:2102905/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:10550/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1392092/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3235810/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3193263/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3120090/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3109886/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1709393/1‑69 (MQ=255) ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:227830/1‑69 (MQ=255) | GTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAC > minE/931662‑931787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |