| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 932,920 | T→C | 63.7% | A139A (GCT→GCC) | fdnG → | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 932,920 | 0 | T | C | 63.7% | 33.9 / 30.6 | 55 | A139A (GCT→GCC) | fdnG | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base T (8/12); new base C (23/12); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGG > minE/932885‑932988 | gaagCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAg < 1:1131121/69‑1 (MQ=255)gaagCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAg < 1:1389734/69‑1 (MQ=255)gaagCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAg < 1:910470/69‑1 (MQ=255)gaagCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAg < 1:1930266/69‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:1040921/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:3369292/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:3214593/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:2842310/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:2499608/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:2358301/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:2074575/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:2048451/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:790887/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:1693344/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:879677/66‑1 (MQ=255) ccGTATTGCGAAGCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGt < 1:863015/66‑1 (MQ=255) aTTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTa < 1:1196379/69‑1 (MQ=255) aTTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTa < 1:3416980/69‑1 (MQ=255) aTTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTa < 1:2634076/69‑1 (MQ=255) aTTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTa > 1:2469241/1‑69 (MQ=255) aTTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTa < 1:2409907/69‑1 (MQ=255) aTTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTa < 1:491382/69‑1 (MQ=255) ttGCGAAGCTGATGAAAGCTGCCCGTGACGCTAACTTTATTGAAAAg > 1:382574/1‑47 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa < 1:2410621/69‑1 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa > 1:3383686/1‑69 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa > 1:2917339/1‑69 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa < 1:1349736/69‑1 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa > 1:1350451/1‑69 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa > 1:1479330/1‑69 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa > 1:990405/1‑69 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa < 1:1969604/69‑1 (MQ=255) ttGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTaa > 1:856104/1‑69 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGa > 1:664485/1‑41 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:434080/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:526166/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:687689/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:904682/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:3431626/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:809056/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:1128960/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:316251/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:3161279/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:2526276/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:2413681/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:2312340/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:2234901/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:2046107/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:2042261/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:1892956/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:1761751/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:1691285/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:1680135/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:1614503/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGtt > 1:158754/1‑68 (MQ=255) gCTGATGAAAGCCGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGt > 1:2460530/1‑67 (MQ=255) tGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCgg > 1:219546/1‑69 (MQ=255) | GAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGG > minE/932885‑932988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |