| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 934,499 | T→G | 50.6% | S666A (TCC→GCC) | fdnG → | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 934,499 | 0 | T | G | 50.6% | 15.8 / 97.7 | 91 | S666A (TCC→GCC) | fdnG | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base T (12/33); new base G (16/30); total (28/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCGTAACGACGGTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACG > minE/934439‑934562 | gcgcGTAACGACGGTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAggt > 1:2637224/1‑69 (MQ=255)gcgcGTAACGACGGTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAggt > 1:2309128/1‑69 (MQ=255)gcgcGTAACGACGGTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAggt > 1:846587/1‑69 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGc > 1:302222/4‑66 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGGGt > 1:1297634/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCg > 1:977448/4‑67 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCg > 1:954667/4‑67 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCg > 1:1719993/4‑67 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:997271/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:2462912/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:2148009/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:2030791/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:1917533/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:759667/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:1301322/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:1129024/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:1524474/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:1204255/4‑68 (MQ=255) ggcgAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGt > 1:1389221/4‑68 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:3231150/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:3098124/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:3138512/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:2072588/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:1971399/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:3167699/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:2736121/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:519538/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:950144/69‑1 (MQ=255) gTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAg < 1:2467365/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGGGTAGAAc < 1:3005042/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:880188/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:2794040/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:1187336/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:2722777/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:3024125/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:136190/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:2026929/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:3406568/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:1641158/69‑1 (MQ=255) aaaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAAc < 1:915511/69‑1 (MQ=255) aaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGTAGAAcc < 1:3019962/69‑1 (MQ=255) aaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGTAGAAcc < 1:3242777/69‑1 (MQ=255) aaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGTAGAAcc < 1:953246/69‑1 (MQ=255) aaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGTAGAAcc < 1:2697075/69‑1 (MQ=255) aaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGTAGAAcc < 1:2658224/69‑1 (MQ=255) aaTTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGGCCGAGGGTGGTAAAGGCGTAGAAcc < 1:1740051/69‑1 (MQ=255) gggTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCtgatg < 1:680833/67‑1 (MQ=255) gggTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCtgatg < 1:3306369/67‑1 (MQ=255) gggTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCtgatg < 1:3315767/67‑1 (MQ=255) gggTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCtgatg < 1:2091790/67‑1 (MQ=255) gggTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCtgatg < 1:2249819/67‑1 (MQ=255) tCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCt < 1:788480/58‑1 (MQ=255) tCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCt < 1:3226595/58‑1 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:2811959/1‑69 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:789668/1‑69 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:2206826/1‑69 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:469520/1‑69 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:2753273/1‑69 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:1543482/1‑69 (MQ=255) tACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGc > 1:1929208/1‑69 (MQ=255) atcatcTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTgg < 1:1683055/68‑1 (MQ=255) catcTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAAc > 1:1084388/1‑69 (MQ=255) catcTGCGCAAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAAc > 1:729644/1‑69 (MQ=255) ccAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACg < 1:3434595/68‑1 (MQ=255) ccAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACg < 1:134338/68‑1 (MQ=255) ccAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACg < 1:406460/68‑1 (MQ=255) ccAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACg < 1:2103808/68‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACGGCTGATAAAGATGAGCt < 1:2225052/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTTATAAAGATGAGCt < 1:1936937/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:941617/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1406085/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1397967/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1598052/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:123103/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:984299/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:763348/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1108655/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1716826/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:562380/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:527080/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:498013/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:37093/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:344884/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1785478/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:3258191/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:1914147/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:2073341/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:2093222/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:2219233/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:2885722/47‑1 (MQ=255) ccAGGCCGAGGGTGGTAAAGGCGTAGAACCGCTGATAAAGATGAGCt < 1:2446938/47‑1 (MQ=255) | GCGCGTAACGACGGTGAAATTCTGGCGGGTATCTACCATCATCTGCGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGGAACTACAAGCAGCCGCACG > minE/934439‑934562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |