| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 83,976 | G→A | 53.8% | G278G (GGG→GGA) | ftsW → | integral membrane protein involved in stabilising FstZ ring during cell division |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 83,976 | 0 | G | A | 53.8% | 9.1 / 79.6 | 93 | G278G (GGG→GGA) | ftsW | integral membrane protein involved in stabilising FstZ ring during cell division |
| Reads supporting (aligned to +/- strand): ref base G (28/15); new base A (39/11); total (67/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGC > minE/83913‑84044 | cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2742598/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1707862/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1976847/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1993843/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2022332/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2091244/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:213836/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2402882/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2449906/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2689384/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1032817/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2863094/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:2988065/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:3126020/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:3163902/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:3184084/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:645831/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:828528/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:888530/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:992039/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1621978/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1377736/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAgg > 1:1041866/1‑69 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAg > 1:2321157/1‑68 (MQ=255)cTTTGGCAGCGGCTATCAGTTAACGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAg > 1:3214470/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGt > 1:1387973/1‑67 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:3028158/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:2970635/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:393344/1‑68 (MQ=255) aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa > 1:2172894/1‑68 (MQ=255) tAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACaaa < 1:1304616/69‑1 (MQ=255) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:1128627/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:1237706/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:2482609/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:588438/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:236951/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:2821219/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:626565/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:3190245/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:201992/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGGTGAACTGTGGGGACAAGGttt < 1:455061/51‑1 (MQ=37) aaCGCAATCGTTGATGGCGTTTGGTCGCGATGAACTGTGGGGACAAGGttt < 1:2628487/51‑1 (MQ=255) tCGCTGATGGGGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGa > 1:1587064/1‑68 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:3099613/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:2610414/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:1098543/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:1348281/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:588596/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:114181/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:2131759/1‑69 (MQ=255) tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAg > 1:69436/1‑69 (MQ=255) gCTGATGGCGTTTTGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:340490/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:1346005/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3038392/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2397804/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3119967/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2452635/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2436548/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2238389/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2143900/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:978898/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3287423/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:3385140/69‑1 (MQ=255) gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:1864786/69‑1 (MQ=255) cTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa < 1:2488172/68‑1 (MQ=255) ttGGTCTCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:5261/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGTTACAAAAACTGGAGTATCTGCCGGAAg > 1:2322288/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1079741/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1802276/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1807511/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1906635/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1958668/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:3348674/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1553271/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:2997836/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:2201737/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1352081/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:2625173/1‑69 (MQ=255) ttGGTCGCGGTGAACTGTGGGGACAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1298716/1‑69 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGc > 1:549283/1‑62 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:71295/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1016419/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:491518/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:339005/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:3126400/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:2604186/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1371736/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:2093404/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1536738/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1974028/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1788525/1‑68 (MQ=255) tGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg > 1:1663652/1‑68 (MQ=255) gcggcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGca > 1:2928866/1‑67 (MQ=255) gCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGc < 1:1300438/69‑1 (MQ=255) gCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGc < 1:956975/69‑1 (MQ=255) | CTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGC > minE/83913‑84044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |