| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 935,197 | C→G | 49.5% | T898T (ACC→ACG) | fdnG → | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 935,197 | 0 | C | G | 49.5% | ‑2.3 / 119.1 | 95 | T898T (ACC→ACG) | fdnG | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base C (34/14); new base G (27/20); total (61/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
AACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTG > minE/935130‑935261 | aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGCCCTATCGTCTGACCg > 1:196294/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:3155182/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:2488139/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:2610946/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:2753512/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:304368/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:126644/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:3293589/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:39840/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:583565/1‑69 (MQ=255)aaCAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:1128779/1‑69 (MQ=255) aGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCa < 1:3004884/69‑1 (MQ=255) aGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCa < 1:2863952/69‑1 (MQ=255) gACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCAc < 1:1159332/69‑1 (MQ=255) gACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCAc < 1:3411538/69‑1 (MQ=255) gACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCAc < 1:886017/69‑1 (MQ=255) gACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCAc < 1:1927041/69‑1 (MQ=255) gcgcTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTc < 1:2690176/69‑1 (MQ=255) gcgcTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTc < 1:1018229/69‑1 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCg > 1:1637233/1‑51 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:2348531/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:3185968/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:2680829/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:705029/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:2166546/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:109469/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:1114005/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:1218512/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:1236116/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTgg > 1:1400206/1‑68 (MQ=255) tGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCAAACCTgg > 1:1481178/1‑68 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:1980794/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:973874/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:875122/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:1119242/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:4721/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:366570/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2592750/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:1006481/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2274636/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:195942/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:1984540/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:217364/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:1995174/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2081494/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2112532/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2134397/69‑1 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAg > 1:2899731/1‑68 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAg > 1:3291961/1‑68 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAg > 1:1412137/1‑68 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGc > 1:2345637/1‑69 (MQ=255) aaaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGc > 1:2955324/1‑69 (MQ=255) aaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:1255190/68‑1 (MQ=255) aaaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2757531/68‑1 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGa > 1:3138583/1‑44 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGa > 1:2438041/1‑44 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2052993/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2022447/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:3320258/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:1204126/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:428951/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2071533/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:471810/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:3267883/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2017224/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2015296/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:700092/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:1308513/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2012865/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2240210/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2450336/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2181632/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2253847/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2330751/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2381577/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:1677333/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2544055/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:1548760/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:2884466/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:1408334/1‑45 (MQ=255) aaaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACGGAg > 1:1409815/1‑45 (MQ=255) aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACg < 1:1847764/69‑1 (MQ=255) aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACg < 1:806662/69‑1 (MQ=255) aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACg < 1:867353/69‑1 (MQ=255) aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACg < 1:2318257/69‑1 (MQ=255) aaGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACg < 1:901830/69‑1 (MQ=255) agagCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACg < 1:730825/68‑1 (MQ=255) cccGTATGTGGGTACGACCTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2429758/56‑1 (MQ=255) cccGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCaa > 1:408476/1‑69 (MQ=255) ccTATCGTCTGACGGAGCACTTCCACACCTGGACCAAGc < 1:2990790/39‑1 (MQ=255) tCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACa > 1:1378535/1‑69 (MQ=255) tCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACa > 1:729654/1‑69 (MQ=255) tCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACa > 1:2551608/1‑69 (MQ=255) tCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACCCATTGCTCAACGCAATTGCTCAGCCGGAACa > 1:2466432/1‑69 (MQ=255) tGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTtg > 1:2852839/1‑69 (MQ=255) | AACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTG > minE/935130‑935261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |