| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 936,797 | G→A | 22.1% | S119S (TCG→TCA) | fdnI → | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 936,797 | 0 | G | A | 22.1% | 245.4 / 52.7 | 171 | S119S (TCG→TCA) | fdnI | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base G (62/71); new base A (26/12); total (88/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 172 total aligned reads displayed.
AGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTA > minE/936731‑936865 | aGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGAt < 1:2928120/69‑1 (MQ=255) gCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:2151249/1‑68 (MQ=255) gCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:436594/1‑68 (MQ=255) gCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:258695/1‑68 (MQ=255) tGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAg < 1:2450887/69‑1 (MQ=255) gAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGc < 1:1960626/69‑1 (MQ=255) gAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGc < 1:2730579/69‑1 (MQ=255) gAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGc < 1:2855349/69‑1 (MQ=255) aGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGc > 1:1008900/1‑68 (MQ=255) tAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:2251479/1‑58 (MQ=255) tAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:2322044/1‑58 (MQ=255) tAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:2063400/1‑58 (MQ=255) tAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:200791/1‑58 (MQ=255) tAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATc > 1:1704934/1‑58 (MQ=255) aaaGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAg < 1:2909739/62‑1 (MQ=255) aaaGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAg < 1:1698938/62‑1 (MQ=255) aaGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGAttt > 1:1063672/1‑69 (MQ=255) aaGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGAttt > 1:3423862/1‑69 (MQ=255) aaGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGAttt > 1:322777/1‑69 (MQ=255) aaGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGAtt > 1:1336245/1‑68 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGtgct < 1:2313087/69‑1 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGtgct < 1:2577381/69‑1 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGtgct < 1:2542588/69‑1 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGttct > 1:1582631/1‑69 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGtgct > 1:1425182/1‑69 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGtgct > 1:2798409/1‑69 (MQ=255) ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGtgct > 1:2110942/1‑69 (MQ=255) tGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTgctgct < 1:1837421/69‑1 (MQ=255) tGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTgctgct > 1:3372035/1‑69 (MQ=255) tGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTgctgct > 1:3157596/1‑69 (MQ=255) tGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTgctgct > 1:2702227/1‑69 (MQ=255) tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1439101/1‑69 (MQ=255) tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:3035871/1‑69 (MQ=255) tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2457304/1‑69 (MQ=255) tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1752189/1‑69 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2617401/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:79657/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2661393/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2913962/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1036188/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2177211/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2038690/1‑68 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:757075/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:653844/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:691982/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:1068415/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:2033567/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:232506/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATg < 1:2106053/43‑1 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGt > 1:2645247/1‑69 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACAGGGGt > 1:176011/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1369050/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:31871/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:2981727/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1168608/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1444764/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:3256345/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:3263424/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1515590/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1550136/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:171127/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:2630346/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:666398/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:243821/1‑69 (MQ=255) cGCCGGGCAAAAGATGATATTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:862254/1‑69 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:569020/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:532838/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:621179/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:1206162/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:3010799/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:1666512/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:2972919/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTTCTGGTGACCGGGGTGATTATCTg < 1:1141148/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:1455318/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:604910/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:2968661/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:308241/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:1236106/69‑1 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:773253/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:2562800/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:3316805/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:1097634/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:401671/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:1713775/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg > 1:3126897/1‑68 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:509704/68‑1 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTATCGTGCTGCTGGTg > 1:2742601/1‑51 (MQ=255) aGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTc < 1:287819/69‑1 (MQ=255) atgatgTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCg < 1:4748/69‑1 (MQ=255) cGATCATGAGCATGATTTTGGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2750882/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2788064/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2281167/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1081168/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1873666/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:138261/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:3191152/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1883471/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:829083/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2408709/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:929835/68‑1 (MQ=255) | AGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTA > minE/936731‑936865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |