| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 936,833 | G→A | 34.0% | G131G (GGG→GGA) | fdnI → | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 936,833 | 0 | G | A | 34.0% | 69.2 / 47.1 | 88 | G131G (GGG→GGA) | fdnI | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base G (21/37); new base A (18/12); total (39/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
TACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGCAGGTTGTTCGC > minE/936765‑936887 | tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1972140/1‑69 (MQ=255)tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2457304/1‑69 (MQ=255)tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1752189/1‑69 (MQ=255)tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2627600/1‑69 (MQ=255)tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2722918/1‑69 (MQ=255)tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1439101/1‑69 (MQ=255)tACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:3035871/1‑69 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2177211/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2038690/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:255210/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:172453/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2617401/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2661393/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:2913962/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:1036188/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:79657/1‑68 (MQ=255) aCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCggg > 1:657683/1‑68 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTTCTGGTGACCGGGGt > 1:829673/1‑69 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGt > 1:2645247/1‑69 (MQ=255) cAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACAGGGGt > 1:176011/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1444764/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:2321300/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:243821/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:743311/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1104724/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:666398/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:2562863/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1168608/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:171127/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:2630346/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:3263424/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1515590/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:1369050/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:3256345/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:2981727/1‑69 (MQ=255) cGCCGGGCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:31871/1‑69 (MQ=255) cGCCGGGCAAAAGATGATATTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGAt > 1:862254/1‑69 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:3423384/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:532838/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:3010799/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:569020/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:2976192/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:2972919/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:621179/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:2774501/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:1666512/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:2527304/69‑1 (MQ=255) gggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGAGTGATTATc < 1:1206162/69‑1 (MQ=255) ggCAAAAGATGATGTTCTGGTCAATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGATTGATTATc < 1:2169753/68‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTTCTGGTGACCGGGGTGATTATCTg < 1:1141148/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:1236106/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:308241/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:2968661/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:1455318/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:2439790/69‑1 (MQ=255) gCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:604910/69‑1 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:1713775/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:401671/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:3316805/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:2056914/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:55730/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:2079369/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:2562800/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:1705270/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:2743105/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:1097634/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:773253/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:1485734/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTgg > 1:2652830/1‑69 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg > 1:3126897/1‑68 (MQ=255) cAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:509704/68‑1 (MQ=255) aGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTc < 1:2023336/69‑1 (MQ=255) aGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTc < 1:1712804/69‑1 (MQ=255) aGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTc < 1:287819/69‑1 (MQ=255) atgatgTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCg < 1:3137687/69‑1 (MQ=255) atgatgTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCg < 1:3191467/69‑1 (MQ=255) atgatgTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCg < 1:4748/69‑1 (MQ=255) tgTTCTTGTCGATGATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:1381294/58‑1 (MQ=38) cGATCATGAGCATGATTTTGGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2750882/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:634768/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1081168/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:829083/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1065336/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:3191152/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2281167/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2312204/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2408709/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1883471/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1873666/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1829736/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:2788064/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1447619/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:1402148/68‑1 (MQ=255) cGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAg < 1:138261/68‑1 (MQ=255) gATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTa < 1:1690821/69‑1 (MQ=255) aGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCg > 1:1085605/1‑69 (MQ=255) tGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGc > 1:3212541/1‑69 (MQ=255) aTTTTCGTGCTGCTGGTGACCGGGGTGATTATCTg < 1:2856570/35‑1 (MQ=255) ctgctgGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGCAGGTTGTTCGc < 1:1648568/69‑1 (MQ=255) ctgctgGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGCAGGTTGTTCGc < 1:703294/69‑1 (MQ=255) | TACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGCAGGTTGTTCGC > minE/936765‑936887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |