| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 937,004 | A→G | 37.5% | V188V (GTA→GTG) | fdnI → | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 937,004 | 0 | A | G | 37.5% | 79.5 / 92.9 | 141 | V188V (GTA→GTG) | fdnI | formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base A (60/28); new base G (30/23); total (90/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
CATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAA > minE/936945‑937069 | cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:3105946/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:3170329/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:270125/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:638417/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:2357464/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:2330464/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:1712234/1‑69 (MQ=255)cATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGc > 1:137240/1‑69 (MQ=255) tGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTgg > 1:2471052/1‑68 (MQ=255) ggCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCgaaga > 1:140437/1‑68 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:3056682/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1922723/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:2039018/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:413847/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:19823/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:3352026/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1934809/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1525232/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1475329/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:478255/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:2180486/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1324504/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1154402/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:2651092/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:1007212/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgccgtc > 1:975815/1‑56 (MQ=255) ggCATTCTGGGTAAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTGAgacgtc > 1:2920305/1‑56 (MQ=255) ttttGGGTGAAAGGATCGATTTAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAactc < 1:3233799/69‑3 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:935737/69‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:803549/69‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:27956/69‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:868010/69‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:2352691/69‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:1392384/69‑1 (MQ=255) ttttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:1567370/69‑1 (MQ=255) tttGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac < 1:303553/68‑1 (MQ=255) gTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCcg > 1:588774/1‑69 (MQ=255) gTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCcg > 1:2910739/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAgtc > 1:378747/1‑37 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAacac > 1:3147728/1‑57 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg < 1:3086615/69‑1 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:1561471/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:2973107/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:227236/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:531296/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:446697/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:2884920/1‑69 (MQ=255) ggATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTgg > 1:797576/1‑69 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACAcc > 1:2650669/1‑51 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACAcc > 1:1257100/1‑51 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:3412845/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:2608088/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:1238660/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:1830769/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:2569736/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:1955143/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa < 1:1052522/52‑1 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:206580/1‑52 (MQ=255) ttAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCa > 1:2242485/1‑52 (MQ=255) aaaGGGATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:129321/69‑1 (MQ=255) aaaGGGATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:2749816/69‑1 (MQ=255) aaaGGGATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:3161966/69‑1 (MQ=255) aaaGGGATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:2047419/69‑1 (MQ=255) aaaGGGATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:685308/69‑1 (MQ=255) aaGGGATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:596273/68‑1 (MQ=255) aGGGATGGTCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaaa < 1:1437216/68‑1 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaaa < 1:1161646/68‑1 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaaa < 1:1281166/68‑1 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaaa < 1:909047/68‑1 (MQ=255) aGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaaa < 1:1825054/68‑1 (MQ=255) gggATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATc < 1:1132521/69‑1 (MQ=255) gggATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATc < 1:1894339/69‑1 (MQ=255) gggATGATCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATc < 1:640764/69‑1 (MQ=255) ggATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCg < 1:17976/69‑1 (MQ=255) ggATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCg < 1:2818495/69‑1 (MQ=255) gatgatCGAAGGGAAGGTAAGTCGTCGCTTGGCGAAGAAACACCATCCGCGCTGGt < 1:1022099/56‑1 (MQ=255) atgatCGAAGGGAAGGTGAGCCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGaa < 1:2306617/63‑1 (MQ=255) tCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTTGTATCTTGAAATCGAGaa > 1:1674222/1‑67 (MQ=255) tCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGaa > 1:1043764/1‑67 (MQ=255) tCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGaa > 1:2963635/1‑67 (MQ=255) tCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGaa > 1:1971857/1‑67 (MQ=255) tCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGaa > 1:2369049/1‑67 (MQ=255) tCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGaa > 1:2223220/1‑67 (MQ=255) gggaaggTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGaa > 1:94259/1‑69 (MQ=255) gaaggTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAg > 1:341436/1‑68 (MQ=255) tAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:750213/1‑67 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTAt > 1:2436180/1‑40 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAAt > 1:1622733/1‑48 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:3248613/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:48311/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:363142/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:2090233/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:3035103/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:3007965/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:2922942/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:2876266/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:2841681/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:245188/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:2726360/1‑66 (MQ=255) aaGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa > 1:2493233/1‑66 (MQ=255) | CATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAA > minE/936945‑937069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |