| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 938,456 | C→T | 28.6% | G162G (GGG→GGA) | adhP ← | alcohol dehydrogenase, 1‑propanol preferring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 938,456 | 0 | C | T | 28.6% | 99.9 / 39.7 | 95 | G162G (GGG→GGA) | adhP | alcohol dehydrogenase, 1‑propanol preferring |
| Reads supporting (aligned to +/- strand): ref base C (22/45); new base T (8/19); total (30/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
CATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTG > minE/938392‑938519 | cATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTg < 1:146260/69‑1 (MQ=255)cATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTg < 1:1990399/69‑1 (MQ=255)cATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTg > 1:2583898/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGaa > 1:1083469/1‑68 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGaa > 1:350034/1‑68 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:2321036/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:2820856/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:1407791/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:137788/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:2111067/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:1172527/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:1134827/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:2528674/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:974042/1‑69 (MQ=255) cgcgTATTGCAGGGCGAGGTTACCCAGACCGACAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAt > 1:1973448/1‑69 (MQ=255) cgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttt > 1:1468169/1‑69 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg > 1:535319/1‑59 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTGGACGAAttt > 1:1208521/1‑68 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTGGACGAAttt > 1:2572913/1‑68 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTGGACGAAttt > 1:200366/1‑68 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTGGACGAAttt > 1:779863/1‑68 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTGGACGAAttt > 1:1945055/1‑68 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTGGACGAAttt > 1:1642973/1‑68 (MQ=255) gTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAG‑AATCCACTGTCCTGGACGAAttttt > 1:983111/1‑69 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:1566337/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:2750883/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:2691349/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:909546/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:2137052/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:429037/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:262085/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:476809/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:611363/69‑1 (MQ=255) tATTGCAGGGCGAGGTTACCCAGACCGCCAAGACAGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:3176692/69‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCTAGACCGTAGATAGCAATCCACTGTCCTg < 1:2660392/55‑1 (MQ=39) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1006662/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:2538546/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:494181/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:358373/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:329443/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:264940/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:312950/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1713392/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:2225743/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1529872/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1419878/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:139004/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1185268/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:2220517/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1896085/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:1945707/55‑1 (MQ=255) tGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:137608/55‑1 (MQ=255) gCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGTCCTg < 1:814285/54‑1 (MQ=255) ttACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAAttttt < 1:1010669/54‑1 (MQ=255) tACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCtt > 1:2224801/1‑69 (MQ=255) aCCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCtt > 1:2064359/1‑68 (MQ=255) aCCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCtt > 1:1076852/1‑68 (MQ=255) aCCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCtt > 1:97199/1‑68 (MQ=255) aCCCAGACCGCCAAGACCGTAGATAGCAATCAACTGCCCTGGACGAATTTTTGACAGCTTAACGGCtt > 1:315111/1‑68 (MQ=255) ccAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTg < 1:1289002/68‑1 (MQ=255) ccAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTg < 1:2307561/68‑1 (MQ=255) ccAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTg < 1:21350/68‑1 (MQ=255) ccAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTg < 1:2380034/68‑1 (MQ=255) ccAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTg < 1:2026224/68‑1 (MQ=255) cAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:2947647/69‑1 (MQ=255) cAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:3007560/69‑1 (MQ=255) cAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:2680059/69‑1 (MQ=255) cAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:3317301/69‑1 (MQ=255) cAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:3190572/69‑1 (MQ=255) aCCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:1200136/66‑1 (MQ=255) aCCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:3091288/66‑1 (MQ=255) aCCGCCAAGACCGTAGATAGCAATCCACCGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTa < 1:1006155/66‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:564047/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:2538330/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:2457158/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:907251/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:980460/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:1273352/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:2710520/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:2848733/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:1864484/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:1297826/69‑1 (MQ=255) gCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAAAGGCTTTGTAggtggt < 1:884614/69‑1 (MQ=255) ccAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAggtggt < 1:1522803/68‑1 (MQ=255) gACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTc < 1:2876861/69‑1 (MQ=255) tataTAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGca < 1:1141745/66‑1 (MQ=255) tAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAgg < 1:613388/69‑1 (MQ=255) tAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAgg < 1:2227608/69‑1 (MQ=255) aGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGt < 1:575230/69‑1 (MQ=255) aGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGt < 1:790533/69‑1 (MQ=255) aGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGt < 1:3086601/69‑1 (MQ=255) cACTGGCCTGGACGTATTTTTGACAGCTTATCGGGTTTGTa < 1:972925/41‑1 (MQ=25) cACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAAtgctg > 1:2780884/1‑69 (MQ=255) cACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAAtgctg > 1:1834224/1‑69 (MQ=255) cACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAAtgctg > 1:2421145/1‑69 (MQ=255) | CATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTG > minE/938392‑938519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |