| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 940,715 | G→A | 39.2% | L20L (CTG→TTG) | sfcA ← | malate dehydrogenase, NAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 940,715 | 0 | G | A | 39.2% | 24.3 / 30.4 | 52 | L20L (CTG→TTG) | sfcA | malate dehydrogenase, NAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base G (14/17); new base A (6/14); total (20/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTC > minE/940652‑940769 | tGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAg > 1:1250851/1‑69 (MQ=255) aCGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTAc > 1:1415447/1‑60 (MQ=255) aCGGCGTTCTTCCATGCTGAAGGCACTGCCTTTACTCAACAACGGAAATTCCAGCAGTAc > 1:1219284/1‑60 (MQ=255) gCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGTAGTACAGGGCCAGCGTa < 1:2003010/69‑1 (MQ=255) gCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTa < 1:1226528/69‑1 (MQ=255) gCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTa < 1:1227966/69‑1 (MQ=255) tcttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAggg < 1:1851835/69‑1 (MQ=255) tcttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAggg < 1:1182075/69‑1 (MQ=255) tcttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAggg < 1:3178454/69‑1 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACTGAAATTCCAGCAGTACAGGGCCAGCGTAAggg > 1:470538/1‑68 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACTGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGa > 1:1457852/1‑69 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:1971650/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:284493/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:2934446/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:21577/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:265285/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:2434362/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:548668/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:691446/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:1173506/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:767847/1‑49 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGa < 1:2697870/69‑1 (MQ=255) cttcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGa > 1:1146007/1‑69 (MQ=255) cttcCATGCAGAAGGCACTGCCTTTATTCAACAACGGAAATTCcagcag > 1:44997/1‑49 (MQ=255) gAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCg < 1:3366235/68‑1 (MQ=255) gAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCg < 1:3226474/68‑1 (MQ=255) cACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCt > 1:2255138/1‑69 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTg > 1:405617/1‑68 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:2994491/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:3062568/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt > 1:866034/1‑69 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt > 1:2616406/1‑69 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt > 1:2597628/1‑69 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:2231301/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt > 1:1430252/1‑69 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt > 1:2070275/1‑69 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:1156004/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:1881948/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:1224468/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt < 1:163464/69‑1 (MQ=255) cTGCCTTTATTCAACAACGGAAATTCAAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGt > 1:1550251/1‑69 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:1336702/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:1363000/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:678133/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:3301453/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:3297450/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:3249817/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:1800713/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:3000495/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:1813153/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:1921271/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:2807266/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:2698897/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:2678357/39‑1 (MQ=255) ccTTTATTCAACAACGGAAATTCCAGCAATACAGGGCCa < 1:2619930/39‑1 (MQ=255) aTTCAACAACGGAAATTCCAGCAATACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTtttttgt > 1:758693/1‑69 (MQ=255) aTTCAACAACGGAAATTCCAGCAATACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTtttttgt > 1:1131435/1‑69 (MQ=255) aTTCAACAACGGAAATTCCAGCAATACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTtttttgt > 1:539322/1‑69 (MQ=255) aTTCAACAACGGAAATTCCAGCAATACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTtttttgt > 1:1553231/1‑69 (MQ=255) aTTCAACAACGGAAATTCCAGCAATACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTtttttgt > 1:2897027/1‑69 (MQ=255) aTTCAACAACGGAAATTCCAGCAATACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTtttttgt > 1:2573294/1‑69 (MQ=255) ttCTACAACGGAAATTCCAGCATTACAGGGCCAGCGTa < 1:3248237/38‑1 (MQ=38) cGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTc < 1:1707213/69‑1 (MQ=255) | TGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTC > minE/940652‑940769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |