| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 941,252 | A→C | 29.9% | V44V (GTT→GTG) | bdm ← | biofilm‑dependent modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 941,252 | 0 | A | C | 29.9% | 125.8 / 86.0 | 124 | V44V (GTT→GTG) | bdm | biofilm‑dependent modulation protein |
| Reads supporting (aligned to +/- strand): ref base A (43/44); new base C (20/17); total (63/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCTGTGCCCGAAGGC > minE/941192‑941316 | gACCACATAATTGATCGTTTGCTGGTAGATATCAGTGAGGATGTCGTTATCAGAAGCTTCAACCCAttt > 1:1047719/1‑69 (MQ=255)gACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCAttt > 1:2810067/1‑69 (MQ=255)gACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCAttt > 1:415186/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCAtt > 1:1116107/1‑59 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCt > 1:968961/1‑68 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:320790/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:1394071/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:1332698/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:2202501/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:379937/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:499342/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:691116/1‑69 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATATGGTCAGCt > 1:857775/1‑68 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:360511/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1033055/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:2910603/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:2366961/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:500697/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:2151604/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:2014306/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1932102/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:177738/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1660053/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1303556/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1332448/69‑1 (MQ=255) cGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1262154/69‑1 (MQ=255) gTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgaga > 1:1625992/1‑69 (MQ=255) gTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgaga > 1:3050806/1‑69 (MQ=255) gTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgaga > 1:1110337/1‑69 (MQ=255) gTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:2681207/68‑1 (MQ=255) gTTTGCTGGGAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgaga > 1:1534419/1‑69 (MQ=255) gATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCAATGAGAATGTCATCtt < 1:3069367/68‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:1840150/69‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:2857808/69‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:1924775/69‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:1963237/69‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:957161/69‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt > 1:2497629/1‑69 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:3196481/69‑1 (MQ=255) atCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:500719/69‑1 (MQ=255) tCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGt < 1:1308985/68‑1 (MQ=255) tGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag > 1:250488/1‑43 (MQ=255) tGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag > 1:810954/1‑43 (MQ=255) tGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag > 1:3071564/1‑43 (MQ=255) tCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGc < 1:2135795/69‑1 (MQ=255) tCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGc < 1:2497962/69‑1 (MQ=255) cGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCt > 1:2161252/1‑69 (MQ=255) cGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCt > 1:2437502/1‑69 (MQ=255) cGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCt > 1:2025763/1‑69 (MQ=255) cGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCt > 1:1203234/1‑69 (MQ=255) gTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGc > 1:3311530/1‑67 (MQ=255) gTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtg > 1:1663525/1‑69 (MQ=255) gTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtg > 1:2687663/1‑69 (MQ=255) gTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtg > 1:2618426/1‑69 (MQ=255) gTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtg > 1:853619/1‑69 (MQ=255) gTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtg > 1:1061356/1‑69 (MQ=255) gTTATCAGAAGCTT‑AACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:374418/69‑1 (MQ=255) tATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:1508864/68‑1 (MQ=255) tATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:1183186/68‑1 (MQ=255) tATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:2890779/68‑1 (MQ=255) tATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:241560/68‑1 (MQ=255) tATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:1453068/68‑1 (MQ=255) tATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:2102371/68‑1 (MQ=255) aTCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCtgt < 1:1927649/67‑1 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:831248/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:670183/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:553837/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:3325881/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:3284543/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:3283332/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:960358/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:1559749/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:2309835/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:150667/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaaca > 1:3155397/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGacaac > 1:936896/1‑52 (MQ=255) aaGCTTCCACCCATTTGGTCAGCTCCATCAGAATGTCATCTACAGTGacaaca > 1:1889363/1‑53 (MQ=255) aaGCTTCCACCCATTTGGTAAGCTACATGAGAATGTCATCGTCAGTGccaac > 1:1385207/1‑52 (MQ=255) aaGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCTGTGCCCGaa > 1:1385202/1‑69 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:790981/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:1711031/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:73557/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:1013652/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:714350/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:909581/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:1496163/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:1060868/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:638551/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:339639/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:322278/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:3218034/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:2603404/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTGCTCTGCCCGAAg < 1:1284248/69‑1 (MQ=255) aGCTTCCACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCGTACTCTGCCCGAAg < 1:1174461/69‑1 (MQ=255) aGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGac > 1:1807025/1‑48 (MQ=255) aGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGac > 1:2692701/1‑48 (MQ=255) cTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCTGTGCCCGAAGGc < 1:3435803/69‑1 (MQ=255) cTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCTGTGCCCGAAGGc < 1:2114382/69‑1 (MQ=255) cTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCTGTGCCAGAAGGc < 1:1267397/69‑1 (MQ=255) ttCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCATTGACAACACCATGCTGTGCCCGAAGGc < 1:2654210/68‑1 (MQ=255) | GACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGTCATCTTCAGTGACAACACCATGCTGTGCCCGAAGGC > minE/941192‑941316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |