| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 942,074 | G→T | 31.8% | G116V (GGC→GTC) | osmC → | osmotically inducible, stress‑inducible membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 942,074 | 0 | G | T | 31.8% | 83.9 / 41.9 | 69 | G116V (GGC→GTC) | osmC | osmotically inducible, stress‑inducible membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (30/17); new base T (14/8); total (44/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAAT > minE/942004‑942131 | tttGCGATTACGAAAATCGCACTGAAGAGTGAAGTT‑‑GGTGCCGGGTATTGATGCCTCTACCTTTGACgg > 1:2861333/1‑69 (MQ=255) cGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGc < 1:1615940/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:2549573/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:2577642/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:1686853/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:1046865/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:417730/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:1126523/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:3244411/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:3233528/68‑1 (MQ=255) gagTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:1570973/68‑1 (MQ=255) gagTGAAGTGGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAg < 1:115243/68‑1 (MQ=255) tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATg > 1:2166612/1‑69 (MQ=255) tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATg > 1:2652564/1‑69 (MQ=255) tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATg > 1:2846349/1‑69 (MQ=255) tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATg > 1:288943/1‑69 (MQ=255) tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATg > 1:517631/1‑69 (MQ=255) tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATg > 1:1039784/1‑69 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:282641/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:2675746/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:752162/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:962412/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:2260118/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:1029490/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:1010657/64‑1 (MQ=255) cgAAGTTGCGGTGCCGGGTATTGATGCCTCCACCTTTGACGTCATTATCCAGAAAGCAAAAGCAg < 1:1616622/64‑1 (MQ=255) aaGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc > 1:144570/1‑69 (MQ=255) aaGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc > 1:1111204/1‑69 (MQ=255) aaGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc > 1:3076243/1‑69 (MQ=255) aaGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc > 1:1491820/1‑69 (MQ=255) aaGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc > 1:2349940/1‑69 (MQ=255) aaGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc > 1:2011076/1‑69 (MQ=255) gTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCg < 1:88688/69‑1 (MQ=255) gTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCg < 1:2746963/69‑1 (MQ=255) ttGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCg < 1:1997914/68‑1 (MQ=255) gCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCa > 1:3247765/1‑69 (MQ=255) gCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCa > 1:1554039/1‑69 (MQ=255) gATGCCTGTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2107983/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2299671/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2065727/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:3402224/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2970330/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2613825/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2154319/1‑69 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGaaagcaaaagca > 1:2273610/1‑39 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCATAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:14000/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:192252/1‑66 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:1628393/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:2891870/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:1043949/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:3000943/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:3226815/1‑68 (MQ=255) tctACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa < 1:796414/68‑1 (MQ=255) ctACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGaa > 1:2392442/1‑68 (MQ=255) ccTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAAt < 1:2246348/67‑1 (MQ=255) ccTTTGACGGCATAATACAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAAt < 1:74027/67‑1 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAgg > 1:1143033/1‑47 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1997681/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2544812/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2484610/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:409635/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1126371/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1263710/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1738066/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2955928/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:777412/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:294075/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2583818/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:958563/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1979962/1‑48 (MQ=255) | TTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAAT > minE/942004‑942131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |