| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 942,108 | C→T | 33.6% | V127V (GTC→GTT) | osmC → | osmotically inducible, stress‑inducible membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 942,108 | 0 | C | T | 33.6% | 64.8 / 40.8 | 80 | V127V (GTC→GTT) | osmC | osmotically inducible, stress‑inducible membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (43/10); new base T (14/13); total (57/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.11e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
GCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACG > minE/942045‑942176 | gCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCa > 1:3247765/1‑69 (MQ=255)gCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCa > 1:1554039/1‑69 (MQ=255) gATGCCTGTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2107983/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2065727/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2613825/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2299671/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:3402224/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2154319/1‑69 (MQ=255) gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:2970330/1‑69 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCATAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:14000/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGaaa > 1:192252/1‑66 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:3000943/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:1628393/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:3226815/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:1043949/1‑68 (MQ=255) gCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGc > 1:2891870/1‑68 (MQ=255) tctACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa < 1:796414/68‑1 (MQ=255) ctACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGaa > 1:2392442/1‑68 (MQ=255) ccTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAAt < 1:2246348/67‑1 (MQ=255) ccTTTGACGGCATAATACAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAAt < 1:74027/67‑1 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAgg > 1:1143033/1‑47 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:409635/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2544812/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2583818/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1997681/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1979962/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2484610/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:958563/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1126371/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:777412/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1738066/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:1263710/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:2955928/1‑48 (MQ=255) tGACGTCATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGt > 1:294075/1‑48 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCGGGTACTGAAAGCGGAAATTACGCTGGAt < 1:2636444/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:2878484/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1141863/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1459944/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1616533/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1644402/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1648286/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1831163/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:529730/67‑1 (MQ=255) gtcATTATCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:2726488/67‑1 (MQ=255) attaTCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:3186729/63‑1 (MQ=255) ttaTCCAGAAAGCAAAAGCAGGATGCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:1468020/63‑1 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:528217/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:2099402/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:1255292/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:1457877/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:3298472/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:1021899/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGa > 1:2927043/1‑50 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCa > 1:3014303/1‑68 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:657816/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:1070177/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:8947/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:2930617/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:1703167/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:176656/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:2826058/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:2804578/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:2654865/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:2053298/1‑69 (MQ=255) aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAg > 1:2151546/1‑69 (MQ=255) agcaaaagcaTGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTg > 1:2777378/1‑52 (MQ=255) agcaaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTg > 1:589608/1‑52 (MQ=255) agcaaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTg > 1:2269081/1‑52 (MQ=255) agcaaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTg > 1:1565959/1‑52 (MQ=255) agcaaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTg > 1:1444445/1‑52 (MQ=255) agcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGAtt < 1:1883774/50‑1 (MQ=255) gcaGGATGCCCGGTCTCTCAGGTTCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:1838105/69‑1 (MQ=255) gcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:3072711/69‑1 (MQ=255) gcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:2915870/69‑1 (MQ=255) gcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:1944080/69‑1 (MQ=255) gcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:1795435/69‑1 (MQ=255) gATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTg > 1:2808290/1‑69 (MQ=255) gATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTg > 1:2212418/1‑69 (MQ=255) gCCCGGTTTCTCAGGTACTGAAAGCGGAAATTACGCTGGAt < 1:2534695/41‑1 (MQ=255) gTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:2227487/57‑1 (MQ=255) ctctcAGGTACTGGAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcg > 1:1845513/1‑69 (MQ=255) ctctcAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcg > 1:1032638/1‑69 (MQ=255) | GCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACG > minE/942045‑942176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |