| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 948,935 | C→A | 35.3% | V634V (GTG→GTT) | dos ← | cAMP phosphodiesterase, heme‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 948,935 | 0 | C | A | 35.3% | 80.3 / 59.4 | 85 | V634V (GTG→GTT) | dos | cAMP phosphodiesterase, heme‑regulated |
| Reads supporting (aligned to +/- strand): ref base C (42/13); new base A (27/3); total (69/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01 | |||||||||||
TGCATTGCATCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTAACTGACGGCAAGCTTCCGCGAT > minE/948873‑949003 | tgcattgcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGAt < 1:3143618/69‑1 (MQ=255)tgcattgcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGAt < 1:2827979/69‑1 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:89570/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:3298363/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:323680/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:3176572/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:3149166/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:3145819/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:285776/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:2684881/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:3416460/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:2314089/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:2165060/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:2010599/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:1961920/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:1407670/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:1426157/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:1102671/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:1236023/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:1180442/1‑69 (MQ=255) gcatCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGcc > 1:508804/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:2261067/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:1017650/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:2020147/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:2835972/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:1222052/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:3182548/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:1380376/1‑69 (MQ=255) cAGATACCTGATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGa > 1:1749792/1‑69 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGTCGGGATATGa > 1:1806132/1‑66 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGaa > 1:634536/1‑67 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGaa > 1:1862288/1‑67 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGaa > 1:141927/1‑67 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGaa > 1:1770848/1‑67 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAata > 1:2843639/1‑69 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAata > 1:2256763/1‑69 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAata > 1:2738563/1‑69 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAata > 1:1786563/1‑69 (MQ=255) tGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAat > 1:2256365/1‑68 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:1725317/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:551769/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:125136/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:2892755/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:330669/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:296130/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:3143944/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:3301473/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:1620361/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:1780168/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:2576892/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:2413977/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:2318926/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:1790447/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:2019015/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:1907217/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:547797/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:197229/1‑48 (MQ=255) gATTAGGCAGCTGATTACTGCGAAAGTGCAGCGCAGACAAGTTAACgg > 1:468293/1‑48 (MQ=255) tGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAatat > 1:3404000/1‑58 (MQ=255) tGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGc < 1:3032982/69‑1 (MQ=255) tGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGc < 1:441573/69‑1 (MQ=255) tGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGc > 1:501837/1‑69 (MQ=255) tGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGc < 1:3382778/69‑1 (MQ=255) tGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGc < 1:2826341/69‑1 (MQ=255) gATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGc < 1:2698493/68‑1 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCAtt > 1:1085672/1‑68 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCAtt > 1:2748241/1‑68 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCAt > 1:3260162/1‑67 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTc > 1:2345520/1‑69 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTc > 1:2123533/1‑69 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTc > 1:1910858/1‑69 (MQ=255) aCTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTc > 1:1789878/1‑69 (MQ=255) gCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTg < 1:3287630/68‑1 (MQ=255) gCGAAAGTGCAGCGCAGACAAGTTAACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTg < 1:1304765/68‑1 (MQ=255) gAAAGTGCAGCTCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCt < 1:844297/68‑1 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCt < 1:1577354/67‑1 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCt < 1:1532897/67‑1 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCt < 1:41496/67‑1 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTaa > 1:1183675/1‑69 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTaa > 1:705303/1‑69 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTaa > 1:729855/1‑69 (MQ=255) aaaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTaa > 1:2899987/1‑69 (MQ=255) aaGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCt < 1:855126/66‑1 (MQ=255) cAGCGCAGACAAGTTAACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTg < 1:392012/59‑1 (MQ=255) cgcCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTAACTGACGGCa < 1:284204/69‑1 (MQ=255) cACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTAACTGACGGCAAGCTTCCGCGAt > 1:501102/1‑69 (MQ=255) | TGCATTGCATCAGACACCTGATTAGGCAGTTGATTACTGCGAAAGTGCAGCGCCGACAAGTTCACGGATAACGCCGGGATATGAATATTCTGGCTACGCCATTCTGCTAACTGACGGCAAGCTTCCGCGAT > minE/948873‑949003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |