| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 949,625 | G→C | 30.0% | P404P (CCC→CCG) | dos ← | cAMP phosphodiesterase, heme‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 949,625 | 0 | G | C | 30.0% | 152.6 / 82.3 | 123 | P404P (CCC→CCG) | dos | cAMP phosphodiesterase, heme‑regulated |
| Reads supporting (aligned to +/- strand): ref base G (35/51); new base C (18/19); total (53/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCA > minE/949559‑949686 | aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACCCCACCGgagaga > 1:3063149/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:3276700/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:1106157/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:856111/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:238484/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:1189140/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:1210437/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:560493/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:2060100/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:2679464/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:3386843/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:1837822/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:2829446/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:1750246/1‑69 (MQ=255) aCGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACCGgagaga > 1:1666321/1‑69 (MQ=255) gCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGCCGGCttt > 1:1770171/1‑69 (MQ=255) gCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCttt > 1:1601497/1‑69 (MQ=255) gCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCttt > 1:2437947/1‑69 (MQ=255) gCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCg > 1:2282241/1‑67 (MQ=255) gCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCg > 1:1276946/1‑67 (MQ=255) caatcaCATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGt > 1:116044/1‑69 (MQ=255) caatcaCATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGt > 1:65296/1‑69 (MQ=255) caatcaCATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGt > 1:1675464/1‑69 (MQ=255) caatcaCATCATGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGt > 1:337570/1‑69 (MQ=255) cacaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCa > 1:2148525/1‑67 (MQ=255) cacaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCa > 1:2841467/1‑67 (MQ=255) cacaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCa > 1:1814460/1‑67 (MQ=255) acaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCg > 1:2509773/1‑69 (MQ=255) acaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCg > 1:745070/1‑69 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:2959549/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:3367749/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:2271332/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:3299757/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:1539888/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:445022/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:1269199/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:1989035/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:69869/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:808173/69‑1 (MQ=255) aTCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:2784063/69‑1 (MQ=255) tCCTTAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:129717/68‑1 (MQ=255) tCCTGAATATGGTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:2197232/68‑1 (MQ=255) aaTATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTc < 1:2922994/57‑1 (MQ=255) atatGGTAAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:2480539/62‑1 (MQ=255) tatGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCTAGGTATTtgt < 1:2137538/69‑1 (MQ=255) atGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATtgt < 1:908123/68‑1 (MQ=255) atGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATtgt < 1:1172965/68‑1 (MQ=255) atGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATtgt < 1:1363410/68‑1 (MQ=255) tGGTCAACACCTATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGc > 1:1904891/1‑69 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTc > 1:2481616/1‑44 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCg > 1:2292812/1‑45 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCg > 1:1483068/1‑45 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCg > 1:669174/1‑45 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCg > 1:605403/1‑45 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGt > 1:2382674/1‑61 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGt > 1:3083356/1‑61 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGt > 1:1825363/1‑61 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGt > 1:1867022/1‑61 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGt > 1:2748717/1‑61 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGt > 1:384762/1‑61 (MQ=255) tGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGc > 1:121074/1‑69 (MQ=255) gTCAACACCGATGAGATACACCACCGGAGAGACGGCTTTGTCGACCAGGTCATCGAg < 1:1850344/57‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:3399854/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:675108/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:3317606/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:598868/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:577990/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:43606/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:98933/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:407922/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:30826/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:1055307/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:1803204/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:1815720/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:188648/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:1965948/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:207328/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:211839/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:2682028/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:3232740/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:2811488/69‑1 (MQ=255) cAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:2876470/69‑1 (MQ=255) gATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCACCGAGGTAATTGTGCAGGt < 1:1131076/62‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:2774513/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:752777/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:1729753/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:1746068/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:2866955/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:3317761/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:3325672/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:2852547/69‑1 (MQ=255) gagaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:2766659/69‑1 (MQ=255) agaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTa < 1:719181/47‑1 (MQ=255) agaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTa < 1:142248/47‑1 (MQ=255) agaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:841798/68‑1 (MQ=255) agaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:2237283/68‑1 (MQ=255) agaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGAtt < 1:1031356/68‑1 (MQ=255) gaTACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGt < 1:1548920/57‑1 (MQ=255) acacCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCa < 1:1839631/69‑1 (MQ=255) acGGGAGAGACGGCTTTGTCGACCAGGGCATCGAGGTAATtgt < 1:2114913/43‑1 (MQ=255) | GGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCA > minE/949559‑949686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |