| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 950,105 | A→T | 27.0% | T244T (ACT→ACA) | dos ← | cAMP phosphodiesterase, heme‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 950,105 | 0 | A | T | 27.0% | 141.2 / 57.9 | 100 | T244T (ACT→ACA) | dos | cAMP phosphodiesterase, heme‑regulated |
| Reads supporting (aligned to +/- strand): ref base A (35/38); new base T (9/18); total (44/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
TGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTA > minE/950038‑950165 | tgcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGaaa > 1:29316/1‑68 (MQ=255)tgcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGaaa > 1:1363648/1‑68 (MQ=255)tgcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGaaa > 1:2984494/1‑68 (MQ=255)tgcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGaaa > 1:1469358/1‑68 (MQ=255) gcACATGGCGGCGTGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:3019002/69‑1 (MQ=255) gcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:401562/69‑1 (MQ=255) gcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:2937796/69‑1 (MQ=255) gcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:295681/69‑1 (MQ=255) gcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:1381073/69‑1 (MQ=255) gcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:3162779/69‑1 (MQ=255) gcACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGt < 1:1589840/69‑1 (MQ=255) ggcggcGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTAc < 1:2007924/69‑1 (MQ=255) ggcggcGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTAc < 1:3325624/69‑1 (MQ=255) gcggcgAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTAc < 1:2090764/68‑1 (MQ=255) gcgAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAgg < 1:417908/69‑1 (MQ=255) gcgAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAgg < 1:3231645/69‑1 (MQ=255) gcgAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAgg < 1:2223975/69‑1 (MQ=255) cgAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAgg < 1:305443/68‑1 (MQ=255) cgAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAgg < 1:717137/68‑1 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTc > 1:1166038/1‑63 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAg > 1:2785020/1‑68 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:2563839/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:2260567/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:3427993/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:2876978/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:2110202/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:875451/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:2081042/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:1883510/1‑69 (MQ=255) ttGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGa > 1:705224/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGa > 1:2201782/1‑68 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:2122124/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:1283537/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:2806684/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:1407879/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:3263500/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:2325594/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:3042303/1‑69 (MQ=255) tGCCTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGAt > 1:1679364/1‑69 (MQ=255) cTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGATgc < 1:2905556/68‑1 (MQ=255) cTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGATgc < 1:2089766/68‑1 (MQ=255) cTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGATgc < 1:2754517/68‑1 (MQ=255) cTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGATgc < 1:1419189/68‑1 (MQ=255) cTTCAAGCTGGCGGATTTGCCTTTCTTCGGTGATATCCGAGAATGTCATTACCAGGTTCTGCAGATgc < 1:2328465/68‑1 (MQ=255) tCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGaaact < 1:1855073/44‑3 (MQ=255) cAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCa > 1:2574235/1‑60 (MQ=255) cAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCa > 1:1812446/1‑60 (MQ=255) cAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCa > 1:1986410/1‑60 (MQ=255) cAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGcgcg > 1:2587904/1‑68 (MQ=255) cAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGcgcg > 1:1230445/1‑68 (MQ=255) cAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGcgcg > 1:1205740/1‑68 (MQ=255) cTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCAc > 1:467994/1‑69 (MQ=255) cTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCAc > 1:1706527/1‑69 (MQ=255) cTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCAc > 1:2483638/1‑69 (MQ=255) cTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCAc > 1:2770031/1‑69 (MQ=255) cTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCAc > 1:1964826/1‑69 (MQ=255) cTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCAc > 1:394926/1‑69 (MQ=255) aaTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATa < 1:1130328/69‑1 (MQ=255) aaTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATa < 1:1477346/69‑1 (MQ=255) aaTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATa < 1:2838490/69‑1 (MQ=255) aaTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATa < 1:2639793/69‑1 (MQ=255) aaTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATa < 1:2107135/69‑1 (MQ=255) aTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATaa < 1:577141/69‑1 (MQ=255) aTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATa < 1:2497709/68‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCa > 1:605198/1‑39 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCa > 1:3137654/1‑39 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:2878506/69‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:746894/69‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:449409/69‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:3433465/69‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:2217538/69‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:2863824/69‑1 (MQ=255) cttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:2648888/69‑1 (MQ=255) ttcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:3079010/68‑1 (MQ=255) tGATATCCGAGAAAGTCATTACCAGGTTGTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATaga > 1:2011508/1‑67 (MQ=255) tGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATaga > 1:2811576/1‑67 (MQ=255) tGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATaga > 1:2991297/1‑67 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:376181/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:2902612/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:812218/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:42041/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:832791/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:3030192/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:2639758/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:2137145/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:2371348/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:1006770/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:949424/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:3088796/60‑1 (MQ=255) tCCGAGAATGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATa < 1:3106306/60‑1 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATgcgc > 1:2295411/1‑35 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:3101030/58‑1 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:964770/58‑1 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:1249142/58‑1 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:2497465/58‑1 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGa < 1:3099754/58‑1 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTa > 1:722875/1‑69 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTa > 1:1634337/1‑69 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTa > 1:1808731/1‑69 (MQ=255) tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTa > 1:3182093/1‑69 (MQ=255) | TGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTA > minE/950038‑950165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |