| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 85,885 | G→A | 41.3% | T127T (ACG→ACA) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 85,885 | 0 | G | A | 41.3% | 30.2 / 51.6 | 75 | T127T (ACG→ACA) | murC | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base G (30/14); new base A (22/9); total (52/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGA > minE/85825‑85944 | tcgtGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaa < 1:3073241/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:3066349/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:3212417/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:1598602/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:774810/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:1537775/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:1403721/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:1407708/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACACGGCAAAACGACa < 1:3329471/69‑1 (MQ=255) gAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACACAAGGCAAAACGACa < 1:2012412/69‑1 (MQ=255) aaTGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAac < 1:2958649/69‑1 (MQ=255) aTGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacc < 1:725070/69‑1 (MQ=255) aTGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacc < 1:2059822/69‑1 (MQ=255) aTGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacc < 1:1215175/69‑1 (MQ=255) gctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccac > 1:800026/1‑69 (MQ=255) gctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccac > 1:2123769/1‑69 (MQ=255) gctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccac > 1:3211866/1‑69 (MQ=255) gctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccac > 1:300477/1‑69 (MQ=255) ctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc > 1:2937936/1‑69 (MQ=255) ctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc > 1:19620/1‑69 (MQ=255) ctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccacc > 1:2001241/1‑69 (MQ=255) ctggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAaccac > 1:1960029/1‑68 (MQ=255) ggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgc > 1:2575560/1‑69 (MQ=255) ggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgc > 1:2008693/1‑69 (MQ=255) ggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgc > 1:2452970/1‑69 (MQ=255) ggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgc > 1:592620/1‑69 (MQ=255) ggctgAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgc > 1:814556/1‑69 (MQ=255) ttAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGtt > 1:1928510/1‑69 (MQ=255) ttAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGtt > 1:1424436/1‑69 (MQ=255) gCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAac < 1:280229/51‑1 (MQ=255) gCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAg < 1:738603/69‑1 (MQ=255) gCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAg < 1:2886001/69‑1 (MQ=255) gCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAg < 1:985012/69‑1 (MQ=255) gCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAg < 1:989245/69‑1 (MQ=255) aTCGCCATTGCCGGAACGCACGGCAAAACGACAac < 1:2433118/35‑1 (MQ=255) gCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCgggg > 1:1014283/1‑69 (MQ=255) gCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCgggg > 1:1790283/1‑69 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGtt < 1:564268/39‑1 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGtt < 1:2166519/39‑1 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGtt < 1:1220883/39‑1 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCGTCTACGCAGAAGCGGGGCTCGa > 1:799994/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:679734/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:2804260/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:1428378/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:1222044/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:1920492/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:1286073/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:918600/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:1765575/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:1633829/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:2885142/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:2369836/1‑68 (MQ=255) gCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGa > 1:524908/1‑68 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCa > 1:3276458/1‑41 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:785147/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:758368/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:1059410/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:1276388/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:524247/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:812216/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:906706/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:646090/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:1450874/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:591617/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:575014/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:1451565/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:1602947/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:3049122/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2006317/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2031961/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2899708/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2137410/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2779422/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2560408/1‑42 (MQ=255) aaCACACGGCAAAACGACAACCACCGCGATGGTTTCCAGCAt > 1:2393718/1‑42 (MQ=255) | TCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGCAGAAGCGGGGCTCGA > minE/85825‑85944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |