| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 964,104 | C→G | 42.0% | R114R (CGG→CGC) | yddA ← | fused predicted multidrug transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 964,104 | 0 | C | G | 42.0% | 19.6 / 49.4 | 50 | R114R (CGG→CGC) | yddA | fused predicted multidrug transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (23/6); new base G (8/13); total (31/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
ATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTT > minE/964044‑964157 | aTCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTaa > 1:2899690/1‑68 (MQ=255)aTCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTaa > 1:752385/1‑68 (MQ=255) tttttATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGgtagta < 1:2502140/68‑1 (MQ=255) ttttATGCTCGACGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGgtagta < 1:1589144/67‑1 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGtt > 1:1379770/1‑66 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTaa > 1:792933/1‑68 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTaa > 1:1036842/1‑68 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTa > 1:1897741/1‑67 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAAc > 1:1479517/1‑69 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAAc > 1:485617/1‑69 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAAc > 1:2015701/1‑69 (MQ=255) tCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAAc > 1:2093053/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTTGCGcc > 1:2504239/1‑68 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGcc > 1:2725512/1‑68 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:353204/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:3418373/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:2609669/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:600487/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:849101/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:201286/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:1556072/1‑69 (MQ=255) cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:1775580/1‑69 (MQ=255) cTGAGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCa > 1:752234/1‑69 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:1024681/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:1280889/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:1304027/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:376583/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:1451864/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:3322077/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:3193474/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:3077288/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:2934111/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:2904722/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:2048841/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:2276110/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:3138565/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:148939/67‑1 (MQ=255) tGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:2463427/67‑1 (MQ=255) agtagtTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTc > 1:3085287/1‑68 (MQ=255) agtagtTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTc > 1:870927/1‑68 (MQ=255) gtagtTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTc > 1:552037/1‑67 (MQ=255) gtagtTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTc > 1:556030/1‑67 (MQ=255) agtTTTTATCTGCAAACCAGCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:1864919/58‑1 (MQ=255) ttttATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCg < 1:902660/55‑1 (MQ=255) tttATCTGCAAACCACCGATTAAGGTAGTAATCTg > 1:290862/1‑35 (MQ=255) tttATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAAc > 1:3218908/1‑40 (MQ=255) tttATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAAc > 1:2655950/1‑40 (MQ=255) tttATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAAc > 1:885649/1‑40 (MQ=255) tttATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAAc > 1:2280813/1‑40 (MQ=255) tttATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGttt > 1:2762836/1‑69 (MQ=255) | ATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTT > minE/964044‑964157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |