| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 974,805 | G→T | 56.2% | intergenic (+557/+435) | lsrB → / ← ydeH | AI2 transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 974,805 | 0 | G | T | 56.2% | 3.3 / 50.9 | 48 | intergenic (+557/+435) | lsrB/ydeH | AI2 transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/11); new base T (8/19); total (18/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACATTTTGTTCGTCAATAAACATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGAAATGCGCGACTCACACCTGC > minE/974741‑974865 | acaTTTTGTTCGTCAATAAACATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTcc > 1:3158191/1‑69 (MQ=255) aaTAAACATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGAc > 1:1883172/1‑69 (MQ=255) aaCATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACcaca > 1:2351791/1‑69 (MQ=255) aaCATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACcaca > 1:776604/1‑69 (MQ=255) aaCATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACcaca > 1:547189/1‑69 (MQ=255) cATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACcaca > 1:2391757/1‑67 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACcac > 1:2620340/1‑61 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:883488/1‑69 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:2425781/1‑69 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:3428549/1‑69 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:1648580/1‑69 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:1639095/1‑69 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:788563/1‑69 (MQ=255) aGCGATTCCTTCCGGTTTGCTTACCCTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCagag > 1:1162221/1‑69 (MQ=255) gCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAgg < 1:431853/69‑1 (MQ=255) gCGATTTCTTCCGGTTTGCGTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAgg < 1:1446844/69‑1 (MQ=255) gCGATTTCTTCCGGGTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAgg < 1:486994/69‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:3324687/39‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:3295447/39‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:1131990/39‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:1316793/39‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:171564/39‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:2192544/39‑1 (MQ=255) tcttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:1743673/39‑1 (MQ=255) cttcCGGTTTGCTTACCCTCATACATTGCCCGGTCCGc < 1:708521/38‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:3385919/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:1029223/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:769161/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:568263/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:157700/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:521988/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:424698/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:3407389/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:3381803/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:1775010/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:3122170/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2983806/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2892199/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2212032/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2573606/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2509560/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2505115/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGa < 1:2325914/57‑1 (MQ=255) cccTCATACATTGCCCTGTCCGCTCTTCCAATGACCACATCC‑‑AGGCTCTTCAGGa < 1:257756/55‑1 (MQ=255) cATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGAAATGCGCGACTCACACctgc > 1:2366667/1‑69 (MQ=255) cATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGAAATGCGCGACTCACACctgc > 1:1717848/1‑69 (MQ=255) cATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGAAATGCGCGACTCACACctg > 1:1209632/1‑68 (MQ=255) cATTGACCGGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGAAATGCGCGACTCACACctgc > 1:1796461/1‑69 (MQ=255) | ACATTTTGTTCGTCAATAAACATGCAGCGATTTCTTCCGGTTTGCTTACCCTCATACATTGCCCGGTCCGCTCTTCCAATGACCACATCCAGAGGCTCTTCAGGAAATGCGCGACTCACACCTGC > minE/974741‑974865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |