| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 975,534 | C→T | 35.0% | V27V (GTG→GTA) | ydeH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 975,534 | 0 | C | T | 35.0% | 83.4 / 62.5 | 111 | V27V (GTG→GTA) | ydeH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (51/21); new base T (22/17); total (73/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 111 total aligned reads displayed.
AGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGTT > minE/975471‑975600 | aGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCAcc > 1:3121903/1‑67 (MQ=255)aGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCa > 1:2040065/1‑68 (MQ=255)aGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCa > 1:620745/1‑68 (MQ=255)aGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCa > 1:2679419/1‑68 (MQ=255)aGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCa > 1:3004731/1‑68 (MQ=255)aGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCa > 1:1221856/1‑68 (MQ=255) gTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACt > 1:2627959/1‑68 (MQ=255) gTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACt > 1:1979414/1‑68 (MQ=255) tATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGt < 1:620449/69‑1 (MQ=255) tATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGt < 1:2124409/69‑1 (MQ=255) tATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGt < 1:1148763/69‑1 (MQ=255) tATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGc < 1:2544601/69‑1 (MQ=255) tATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGc < 1:3179641/69‑1 (MQ=255) tATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGc < 1:602080/69‑1 (MQ=255) ttACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGc < 1:1723505/68‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:391906/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1859257/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2282896/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1110532/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2295678/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2555390/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2593419/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2615665/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:641739/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1186881/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1428924/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1454752/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1513669/69‑1 (MQ=255) tACTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:732261/69‑1 (MQ=255) aCTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2705280/68‑1 (MQ=255) aCTGGCATCTCTCGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:609071/68‑1 (MQ=255) cATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGc < 1:2511629/52‑1 (MQ=255) ctctcGGGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:3012473/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTgg > 1:471256/1‑41 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCa > 1:632335/1‑50 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGa > 1:447853/1‑54 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:755972/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:644451/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:1731584/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:2825186/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:2934410/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:1119948/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:2618422/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:2286836/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:3035012/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:2207157/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:1475240/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:355359/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:1562731/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:1602338/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:587070/1‑68 (MQ=255) ctctcGCGACCACGCTGTGAAACATACTTACCAGCAACTGGTAGTGGGCATCGATAGCCTTATTgaga > 1:3319959/1‑68 (MQ=255) tctcGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGAt > 1:1845008/1‑68 (MQ=255) gcgACCACGCTGTGAAACTTACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2286511/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAg > 1:888975/1‑52 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:3319844/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:892883/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2540398/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2587098/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:2535072/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1147779/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1682186/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCt < 1:1911297/55‑1 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGAttt > 1:2266485/1‑66 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTaa > 1:3063210/1‑68 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:621324/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:2058349/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:371194/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:1710675/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:3430499/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:664507/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:1121798/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:1117675/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTa > 1:1064229/1‑67 (MQ=255) gcgACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAAc > 1:3005683/1‑69 (MQ=255) gACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACaa < 1:2127862/69‑1 (MQ=255) aaaCATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACaa < 1:3235811/57‑1 (MQ=255) aaaCATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACaa < 1:3346198/57‑1 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTcc > 1:3098935/1‑68 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTcc > 1:1901362/1‑68 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTc > 1:1140579/1‑67 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2559713/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2699419/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2496714/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2397175/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2383289/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:1756197/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2281439/1‑69 (MQ=255) tACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCg > 1:2134737/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGTGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:1616368/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:1462103/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:422311/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:1329191/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:128974/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:2828225/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGtt > 1:3357641/1‑69 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGt > 1:153229/1‑68 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGt > 1:2048948/1‑68 (MQ=255) cTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGt > 1:743852/1‑68 (MQ=255) cTCACCAGCCAATGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGt > 1:2539976/1‑68 (MQ=255) | AGAATGGTTATCCGTTATTTCTGGCTTACTGGCATCTCTCGCGACCACGCTGTGAAACATACTCACCAGCCACTGGTAGTGGGCATCGATAGCCTTATTGAGATTTAACAAGATGGCATCAATTTCCGTT > minE/975471‑975600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |