| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 977,140 | T→G | 35.9% | K669Q (AAG→CAG) | dcp ← | dipeptidyl carboxypeptidase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 977,140 | 0 | T | G | 35.9% | 58.4 / 79.8 | 92 | K669Q (AAG→CAG) | dcp | dipeptidyl carboxypeptidase II |
| Reads supporting (aligned to +/- strand): ref base T (28/31); new base G (20/13); total (48/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGG > minE/977073‑977204 | tatCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTa < 1:582138/69‑1 (MQ=255)tatCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTa < 1:1440134/69‑1 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:991024/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:2644237/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:2763823/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:3071915/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:2279783/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:2268136/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:3195469/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:2082143/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:2042010/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:3417143/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:419573/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:1808718/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:639483/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:707223/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:655324/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:838741/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:123151/4‑69 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAg > 1:2441352/4‑68 (MQ=255) atccAGGCCAGCCATTGGTGGTGTTTATACGTTCAAGCCACGACGTTGCAGCATCGGCATAATCTGAgg > 1:3172878/4‑69 (MQ=255) atccAGGCCAGACATTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAgg > 1:498543/4‑69 (MQ=255) agccaTTGGTGGTGTTTATACGTTTAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:2372042/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:1690549/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:2284191/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:985959/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:1179914/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:307273/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:3309619/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:2133516/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:1280886/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:249590/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:2782952/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:87416/69‑1 (MQ=255) agccaTTGGTGGTGTTTATACGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTGAGGTGCCTTAcc < 1:1873280/69‑1 (MQ=255) ttGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:1125326/69‑1 (MQ=255) ttGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:2907230/69‑1 (MQ=255) ttGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:2735423/69‑1 (MQ=255) ttGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:2203875/69‑1 (MQ=255) ttGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:2097734/69‑1 (MQ=255) ttGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:1233445/69‑1 (MQ=255) ggtggtGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCAt > 1:945376/1‑69 (MQ=255) ggtggtGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCAt > 1:2897498/1‑69 (MQ=255) ggtggtGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCAt > 1:402142/1‑69 (MQ=255) ggtggtGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCAt > 1:1078771/1‑69 (MQ=255) gtgtTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc < 1:255846/69‑1 (MQ=255) gtgtTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc < 1:827582/69‑1 (MQ=255) gtTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGa < 1:3203059/69‑1 (MQ=255) gtTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGa < 1:2110130/69‑1 (MQ=255) gtTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGa < 1:2008964/69‑1 (MQ=255) tataTGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:1062428/58‑1 (MQ=255) tataTGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:1620832/58‑1 (MQ=255) tataTGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:1827515/58‑1 (MQ=255) tataTGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGcc < 1:2420964/58‑1 (MQ=255) tGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTc < 1:764160/60‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:1027148/69‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:1143324/69‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:1367119/69‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:2535990/69‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:1719122/69‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:1998291/69‑1 (MQ=255) aTGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:3138966/69‑1 (MQ=255) ttGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:564161/66‑1 (MQ=255) agcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGtt < 1:2702318/68‑1 (MQ=255) agcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGtt < 1:654633/68‑1 (MQ=255) agcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGtt < 1:2965904/68‑1 (MQ=255) agcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGtt < 1:3080607/68‑1 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCt > 1:270003/1‑56 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:2527482/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:1592520/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:231097/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:1948526/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:254745/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:1981662/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:1170292/1‑69 (MQ=255) tCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACctc > 1:2177031/1‑69 (MQ=255) cATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc < 1:2459108/54‑1 (MQ=255) aTAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGaa > 1:1304471/1‑69 (MQ=255) aTCTTAGGTTCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTc > 1:2039787/1‑50 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGc > 1:83856/1‑52 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGaaa > 1:2934772/1‑67 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:1403517/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:2694388/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:614690/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:784783/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:306348/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:2407664/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:462181/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:375173/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:3427661/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:2147738/1‑69 (MQ=255) aTCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAgg > 1:2232100/1‑69 (MQ=255) | TATCTCAGGCCAGCCATTGGTGGTGTTTATATGTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGG > minE/977073‑977204 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |