Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 978,107 C→A 42.3% R346R (CGG→CGT)  dcp ← dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE978,1070CA42.3% 15.2 / 25.8 33R346R (CGG→CGT) dcpdipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base C (4/15);  new base A (12/2);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 3.61e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAATGCCCAGTCC  >  minE/978042‑978140
                                                                 |                                 
tCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCccgc                                <  1:1132394/69‑1 (MQ=255)
tCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCccgc                                <  1:3276647/69‑1 (MQ=255)
tCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCccgc                                <  1:3255404/69‑1 (MQ=255)
tCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCccgc                                <  1:1355139/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:933006/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:3294473/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:2985986/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:2719899/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:2567613/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:2506737/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:2320811/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:1956657/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:1852563/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:1322632/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:1691452/69‑1 (MQ=255)
         acCGTGTTTAATTCAAAATATGGCTTGAGCTACGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:2645641/69‑1 (MQ=255)
               tttAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGc                  >  1:2251063/1‑68 (MQ=255)
               tttAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGc                  >  1:2790798/1‑68 (MQ=255)
                 tAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTTCCGCCGTACCTg                        >  1:2611139/1‑60 (MQ=255)
                   aTTCGAAATAAGGCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATaa              <  1:1675591/68‑1 (MQ=255)
                   aTTCGAAATAAGGCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATaa              <  1:531140/68‑1 (MQ=255)
                   aTTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCccgc                                <  1:2887541/50‑1 (MQ=255)
                   aTTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATaa              <  1:1292093/68‑1 (MQ=255)
                   aTTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATaa              <  1:359857/68‑1 (MQ=255)
                      cAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAATg          >  1:1117210/1‑69 (MQ=255)
                           tatGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGt                       <  1:667854/51‑1 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcg  >  1:1575012/1‑68 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:1554795/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:2575637/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:2801327/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:1607320/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:3246045/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:1249564/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:1203146/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:3397235/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:1633559/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:534255/1‑69 (MQ=255)
                              ggCTTGAGCTGCGCTTCATCGAGATCGAATTTCTCACGTCTTACCTGCTCGGCATAAAATGCCCAGTcc  >  1:90394/1‑69 (MQ=255)
                                                                 |                                 
TCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCTGCGCCTCATCAAGATCAAATTTCTCCCGCCGTACCTGTTCCGCATAAAATGCCCAGTCC  >  minE/978042‑978140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑