Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 987,016 A→C 42.1% I419I (ATA→ATC)  ynfE → oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE987,0160AC42.1% 27.9 / 113.8 107I419I (ATA→ATC) ynfEoxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base A (44/18);  new base C (18/27);  total (62/45)
Fisher's exact test for biased strand distribution p-value = 1.63e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-02

Only 100 of 107 total aligned reads displayed.

GACGGGCAATGTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAT  >  minE/986950‑987079
                                                                  |                                                               
gACGGGCAATGTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:1642243/68‑1 (MQ=255)
gACGGGCAATGTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:2266277/68‑1 (MQ=255)
gACGGGCAATGTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:2673594/68‑1 (MQ=255)
gACGGGCAATGTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:1159801/68‑1 (MQ=255)
           tCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCgg                                                    >  1:923292/1‑69 (MQ=255)
           tCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCgg                                                    >  1:364525/1‑69 (MQ=255)
           tCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCgg                                                    >  1:3052446/1‑69 (MQ=255)
           tCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCg                                                     >  1:2258896/1‑68 (MQ=255)
           tCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCg                                                     >  1:3018501/1‑68 (MQ=255)
           tCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCg                                                     >  1:3234892/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGATCGCTTGCCGGTg                                                  >  1:2361929/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:3354771/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:3397748/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:3434747/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:1906135/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:2058107/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:3158266/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:1644527/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:42134/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:1459199/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:2694390/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:1411106/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:2280347/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:1071032/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:81731/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:880039/1‑68 (MQ=255)
              gCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTACACCATTACCATCGAACGCTTGCCGGTg                                                  >  1:2971573/1‑68 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:40485/49‑1 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:3254277/49‑1 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:1466036/49‑1 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAg                                                                <  1:765395/49‑1 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2786974/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2537457/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2638183/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2774995/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2916048/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:379840/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:53656/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:788588/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:901616/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:988580/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:1733176/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2228971/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:1737155/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:1536816/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:105766/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:2467098/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGAt                                            >  1:1065414/1‑69 (MQ=255)
                   aGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCCGGAt                                            >  1:2061500/1‑69 (MQ=255)
                              tAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCaa                                 <  1:169156/69‑1 (MQ=255)
                              tAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCaa                                 <  1:579026/69‑1 (MQ=255)
                              tAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCaa                                 <  1:1058476/69‑1 (MQ=255)
                              tAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCaa                                 <  1:2402096/69‑1 (MQ=255)
                              tAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCaa                                 <  1:2377472/69‑1 (MQ=255)
                               aGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCaa                                 <  1:2853657/68‑1 (MQ=255)
                                         gTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAAtct                      <  1:2197022/69‑1 (MQ=255)
                                         gTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAAtct                      <  1:899998/69‑1 (MQ=255)
                                                        ccattaccatCTAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:766405/67‑1 (MQ=39)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCTATCTCCTGCTTCAGCTg         <  1:493315/67‑1 (MQ=39)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:3275821/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:1874744/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:3350559/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:316882/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:1660828/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:425602/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:836311/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:629074/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:1286666/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:794600/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:3259493/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:242302/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:1003873/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:2805870/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:2818019/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:2662775/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:2348747/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:969146/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:266191/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:3135185/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:906970/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:260053/67‑1 (MQ=255)
                                                        ccattaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:2057380/67‑1 (MQ=255)
                                                           ttaccatCGAACGCTTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTg         <  1:2703472/64‑1 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGAc      >  1:63166/1‑65 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGa   >  1:2427075/1‑68 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:2526687/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:988268/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:1030613/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:1026674/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:1043737/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:544892/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:1457598/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:398627/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:1737246/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:1890798/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:3142164/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:304256/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:3037859/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:2863771/1‑69 (MQ=255)
                                                             accatAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAt  >  1:2470948/1‑69 (MQ=255)
                                                                  |                                                               
GACGGGCAATGTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAATCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGAT  >  minE/986950‑987079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑