Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 992,778 A→G 28.2% L194L (TTA→TTG dmsD → twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE992,7780AG28.2% 134.8 / 58.2 131L194L (TTA→TTGdmsDtwin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand):  ref base A (62/32);  new base G (14/23);  total (76/55)
Fisher's exact test for biased strand distribution p-value = 5.50e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

Only 100 of 135 total aligned reads displayed.

CACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGT  >  minE/992710‑992836
                                                                    |                                                          
cacCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTa                                                            >  1:2081675/1‑69 (MQ=255)
cacCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTa                                                            >  1:1666220/1‑69 (MQ=255)
cacCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACAGTTa                                                            >  1:262688/1‑69 (MQ=255)
                cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTa                                            >  1:2443601/1‑69 (MQ=255)
                cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTa                                            >  1:2896523/1‑69 (MQ=255)
                            tGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTc                                <  1:482455/69‑1 (MQ=255)
                            tGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTc                                <  1:246047/69‑1 (MQ=255)
                            tGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTc                                <  1:1173273/69‑1 (MQ=255)
                            tGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTc                                <  1:1763002/69‑1 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgt                          <  1:1349010/67‑2 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:2794367/67‑1 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:151269/67‑1 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:2488797/67‑1 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:2703054/67‑1 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:11646/67‑1 (MQ=255)
                                    ttAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:393527/67‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:1942218/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:2079104/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:2022156/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:1051360/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:2128990/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:2132952/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:3188187/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:3249686/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:3415568/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:625358/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:1930680/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:1093066/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:1079259/69‑1 (MQ=255)
                                     tAACGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:1659126/69‑1 (MQ=255)
                                      aaCGCTGGCGCAATGGCAATCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATc                       <  1:882802/68‑1 (MQ=255)
                                        cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     <  1:2559847/68‑1 (MQ=255)
                                          cTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGt                  <  1:3015127/69‑1 (MQ=255)
                                            ggCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCTCCGTaa                <  1:442519/69‑1 (MQ=255)
                                            ggCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTaa                <  1:3091196/69‑1 (MQ=255)
                                             gcgcAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTaa                <  1:316329/68‑1 (MQ=255)
                                                   tGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAgg                          <  1:2110579/52‑1 (MQ=255)
                                                     gCAATCACAACTGTTAATTTCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCgg       >  1:884300/1‑69 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCa                      >  1:1818657/1‑54 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAcc                    >  1:2303941/1‑56 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:697173/1‑55 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:3046219/1‑55 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:599300/1‑55 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:325399/1‑55 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:1838703/1‑55 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:461307/1‑55 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:1027311/1‑57 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:1420814/1‑57 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:1266585/1‑57 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:261392/1‑57 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGt                  >  1:2660466/1‑58 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCgg       >  1:96290/1‑69 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCg        >  1:2004313/1‑68 (MQ=255)
                                                     gCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATAATGCCgg       >  1:2683713/1‑69 (MQ=255)
                                                       aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGccatg      <  1:1252458/68‑4 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGacg    >  1:89370/1‑67 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:2680654/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:433556/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:1404482/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:2852219/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:2890584/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:1053790/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:2469527/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:3164744/1‑69 (MQ=255)
                                                        aTCACAACTGTTGATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTATTATTGCCGGATg    >  1:2270910/1‑69 (MQ=255)
                                                        aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATg    <  1:2059791/69‑1 (MQ=255)
                                                        aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATg    <  1:220342/69‑1 (MQ=255)
                                                        aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATg    <  1:634857/69‑1 (MQ=255)
                                                        aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATg    <  1:433968/69‑1 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCg                               >  1:3111580/1‑40 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGa                              >  1:1933927/1‑41 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGa                              >  1:298394/1‑41 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGa                              >  1:220280/1‑41 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGa                              >  1:2736568/1‑41 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGa                              >  1:2708127/1‑41 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAcc                    >  1:3049321/1‑51 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:2991258/1‑52 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:3310904/1‑52 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:3344933/1‑52 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:849459/1‑52 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCGGGATGGt  >  1:2685224/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:969981/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:1884446/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:2025541/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:1599454/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:2381666/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:518433/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:2575508/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:1396764/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:361287/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:3391467/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:3385783/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:1126624/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGt  >  1:3191834/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATAGCCGGATGGt  >  1:1538160/1‑69 (MQ=255)
                                                          cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTCTTTCGa                              >  1:1301979/1‑41 (MQ=255)
                                                           acaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCAc                     >  1:1477683/1‑49 (MQ=255)
                                                           acaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCg                   >  1:269663/1‑51 (MQ=255)
                                                           acaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGt                  >  1:84613/1‑52 (MQ=255)
                                                           acaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGt                  >  1:1608423/1‑52 (MQ=255)
                                                                    |                                                          
CACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGT  >  minE/992710‑992836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: