| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 994,058 | A→G | 52.2% | T351T (ACA→ACG) | clcB → | predicted voltage‑gated chloride channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 994,058 | 0 | A | G | 52.2% | 11.7 / 111.5 | 128 | T351T (ACA→ACG) | clcB | predicted voltage‑gated chloride channel |
| Reads supporting (aligned to +/- strand): ref base A (38/23); new base G (45/21); total (84/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
CCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTC > minE/993997‑994123 | ccATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTACCTGATGGCGAAGAAATTACGCTTTTAc > 1:1822003/1‑69 (MQ=255)ccATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTACCTGATGGCGAAGAAATTACGCTTTTAc > 1:193806/1‑69 (MQ=255) ggCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGa > 1:1425821/1‑69 (MQ=255) ggCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGa > 1:2873284/1‑69 (MQ=255) ggCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGa > 1:3200021/1‑69 (MQ=255) aTGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTg < 1:3225273/69‑1 (MQ=255) ttgtATTGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:1144542/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGAt > 1:2181382/1‑64 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:2978255/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:318962/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:652709/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:2730804/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:2589262/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:847561/1‑69 (MQ=255) ttgtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc > 1:2403881/1‑69 (MQ=255) gtATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCgg < 1:1071666/69‑1 (MQ=255) gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATg > 1:2305140/1‑68 (MQ=255) gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATg > 1:2107634/1‑68 (MQ=255) gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATg > 1:3188417/1‑68 (MQ=255) gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATg > 1:279399/1‑68 (MQ=255) gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATg > 1:3208219/1‑68 (MQ=255) gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGA‑TGACCGGGATGGc > 1:1577411/1‑69 (MQ=255) tATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcg > 1:3264866/1‑68 (MQ=255) tATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACAGGGATGGCGACACTGTTggcg > 1:2318898/1‑68 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:1802395/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:2822616/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:2853735/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:3314580/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:1372221/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:3001598/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:2350653/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:1260195/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc > 1:2042201/1‑69 (MQ=255) aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcc > 1:3083059/1‑66 (MQ=255) aTGGTTCCCGGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcgg > 1:2870223/1‑68 (MQ=255) ggTTACCTTATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:3146514/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:1705177/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:717575/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:3099246/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:930959/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:335757/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:1783472/67‑1 (MQ=255) ggTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:2619627/67‑1 (MQ=255) gTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:730870/66‑1 (MQ=255) gTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:1573769/66‑1 (MQ=255) gTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTggcggc < 1:40328/66‑1 (MQ=255) gTTACCTGATGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGAATGGCGACACTGTTggcggc < 1:1798546/66‑1 (MQ=255) tGGCGAAGAAATTACTCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACcacgca > 1:3024267/1‑66 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACcacgcacg > 1:374229/1‑68 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACcacgcacg > 1:264817/1‑68 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:2929006/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:919082/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:3049852/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:1276503/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:1887569/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:1404800/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:3337530/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:2627176/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:2473997/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:24085/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:750832/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:580609/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:2032279/1‑69 (MQ=255) tGGCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:622662/1‑69 (MQ=255) ggCTAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:1768861/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:1324201/1‑68 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCacgcacgc > 1:480982/1‑68 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:349124/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:487101/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:675531/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:1074652/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:2705968/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:2344127/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:2146545/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:2601976/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:201474/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:1666746/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:2770170/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:293601/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:138539/1‑69 (MQ=255) ggCGAAGAAATTACGCTTTTACTCGGATTGACCGGGATGGCGACACAGTTGGCGGCGACCacgcacgc > 1:2489956/1‑68 (MQ=255) gCGAAGAAATTACACTTTTACTCGGATTGACCGGGa > 1:575961/1‑36 (MQ=255) gCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAcgcg > 1:3072413/1‑68 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:159325/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:938831/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:1902919/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:2012663/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:1078395/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:3376981/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:263945/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:2744691/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:3289153/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:3080867/69‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCg < 1:3135079/69‑1 (MQ=255) agaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGAt < 1:2502633/69‑1 (MQ=255) tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTc < 1:2458939/69‑1 (MQ=255) tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTc < 1:3420333/69‑1 (MQ=255) tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTc < 1:190215/69‑1 (MQ=255) tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTc < 1:1727449/69‑1 (MQ=255) tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTc < 1:3164731/69‑1 (MQ=255) | CCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTC > minE/993997‑994123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |