| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 994,518 | T→C | 22.9% | V131V (GTA→GTG) | ynfK ← | predicted dethiobiotin synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 994,518 | 0 | T | C | 22.9% | 112.4 / 26.9 | 87 | V131V (GTA→GTG) | ynfK | predicted dethiobiotin synthetase |
| Reads supporting (aligned to +/- strand): ref base T (31/36); new base C (8/12); total (39/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
AGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGA > minE/994453‑994585 | aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2825644/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:997871/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:3407663/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2633243/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2515559/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2490808/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2483918/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:483206/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2442205/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:411305/69‑1 (MQ=255)aGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:2408520/69‑1 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:209746/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:1128018/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:3213035/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:2406418/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:3154263/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:2980486/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:290491/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:29077/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:2003214/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:682839/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:1761244/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCa > 1:531521/1‑68 (MQ=255) gCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACAGGCAGTTGTTCCTGCACTACCCa > 1:1835891/1‑68 (MQ=255) ggTTAATGCAACCTTCCTGAATACCGACACCCATCAACACCGGCAGTTGTTCCTGCACTACCCAt > 1:265712/1‑65 (MQ=255) ggTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCAtt > 1:26890/1‑66 (MQ=255) ggTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCAtt > 1:1990178/1‑66 (MQ=255) ggTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCAtt > 1:1205478/1‑66 (MQ=255) gTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCaga < 1:1194651/69‑1 (MQ=255) aTACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTAcc < 1:3331623/42‑1 (MQ=255) gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:3029391/68‑1 (MQ=255) gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:856344/68‑1 (MQ=255) gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:53222/68‑1 (MQ=255) gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:420709/68‑1 (MQ=255) gACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATtcatca < 1:2299297/68‑1 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCa > 1:2568230/1‑68 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCa > 1:2066386/1‑68 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:292822/1‑69 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:2309264/1‑69 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:2168533/1‑69 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:2061862/1‑69 (MQ=255) aaCACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAg > 1:3110280/1‑69 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:885758/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:1172492/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:430748/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:3116580/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:380011/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:3249810/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:3400536/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:2813521/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:1443740/69‑1 (MQ=255) ccGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:2184704/69‑1 (MQ=255) ccGGCAGTGGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:1781034/69‑1 (MQ=255) cGGCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAgccgc < 1:2720543/68‑1 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCa > 1:1229926/1‑68 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2796535/1‑69 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:969335/1‑69 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2735370/1‑69 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:888375/1‑69 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2431597/1‑69 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:2211601/1‑69 (MQ=255) gCAGTTGTTCCTGCACCACCCATTCAGAGAGCGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAg > 1:1322687/1‑69 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:1481723/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:1218510/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:1479079/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:568410/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:643021/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:693465/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:1236752/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:979975/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:166063/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:2986783/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:243843/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:3179276/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:3324458/68‑1 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt > 1:2210646/1‑68 (MQ=255) gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCt < 1:3402971/68‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:1306719/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:3300040/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:822771/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:852809/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:2291082/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:86436/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:1804998/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:3017406/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:3010130/69‑1 (MQ=255) cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:1561544/69‑1 (MQ=255) tACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGa < 1:772377/68‑1 (MQ=255) | AGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGA > minE/994453‑994585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |