| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 995,650 | T→A | 40.0% | A202A (GCA→GCT) | dgsA ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 995,650 | 0 | T | A | 40.0% | 19.5 / 40.9 | 45 | A202A (GCA→GCT) | dgsA | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (14/13); new base A (10/8); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
CCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAG > minE/995583‑995712 | ccACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTg < 1:1355555/69‑1 (MQ=255) cACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGc > 1:988553/1‑69 (MQ=255) cACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGc > 1:30913/1‑69 (MQ=255) cACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGc > 1:2639474/1‑69 (MQ=255) cACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGc > 1:1572331/1‑69 (MQ=255) cACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGc > 1:2143177/1‑69 (MQ=255) aCGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGcc < 1:1309439/69‑1 (MQ=255) aCGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGcc < 1:104656/69‑1 (MQ=255) aCGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGcc < 1:199796/69‑1 (MQ=255) aCGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGcc < 1:2051175/69‑1 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:2523630/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:823349/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:724347/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:3067753/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:2714111/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:224069/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:1406287/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:1129978/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:1551663/1‑68 (MQ=255) gtgATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCAGCCATCg > 1:1683581/1‑68 (MQ=255) cTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCATCCATGCGCTGATATc > 1:689809/1‑69 (MQ=255) gAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGACCTCTGCCATCGTCCATGCGCTGATATCAt > 1:2081404/1‑69 (MQ=255) aTCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGaa < 1:2666086/68‑1 (MQ=255) aTCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGaa < 1:2670033/68‑1 (MQ=255) tCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGaa < 1:510121/67‑1 (MQ=255) cccGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCgg < 1:816846/69‑1 (MQ=255) cccGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCgg < 1:289950/69‑1 (MQ=255) ggCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCgg < 1:593992/68‑1 (MQ=255) aaCAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc < 1:1152642/69‑1 (MQ=255) cAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc > 1:1309908/1‑67 (MQ=255) cAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc > 1:2124110/1‑67 (MQ=255) cAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc > 1:667555/1‑67 (MQ=255) cAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc > 1:14091/1‑67 (MQ=255) cAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc > 1:263199/1‑67 (MQ=255) cAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTAtgctgc > 1:2200424/1‑67 (MQ=255) aaGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTc > 1:188507/1‑68 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:3270703/69‑1 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:3076848/69‑1 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:546991/69‑1 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:2946011/69‑1 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:1945784/69‑1 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:1134257/69‑1 (MQ=255) aGGCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:249197/69‑1 (MQ=255) ggCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAg < 1:443402/69‑1 (MQ=255) ggCCTCAGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGTACGCCGGTATGCTGCTCCa < 1:508304/68‑1 (MQ=255) | CCACGTTGTGATCGATAACCACCTGAATCACATCGCGCGCCCCGCGTGAGGCACCAAACAAGGCCTCTGCCATCGTCCATGCGCTGATATCATGCTGAATATAAACCGGAACGCCGGTATGCTGCTCCAG > minE/995583‑995712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |