| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 996,736 | T→C | 42.4% | N183S (AAT→AGT) | ynfL ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 996,736 | 0 | T | C | 42.4% | 29.7 / 82.0 | 106 | N183S (AAT→AGT) | ynfL | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (41/20); new base C (18/27); total (59/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
CAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACT > minE/996669‑996801 | cAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACAt > 1:1711113/1‑68 (MQ=255) gTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTa < 1:1265437/69‑1 (MQ=255) aCAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCggg < 1:1006640/69‑1 (MQ=255) aCAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCggg < 1:492230/69‑1 (MQ=255) aCAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTAGGGTTATTTGCCAGGGGATGATCGTGCggg < 1:870898/69‑1 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:1129677/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:3136704/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:2945630/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:3385036/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:2860511/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:3245049/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:2853069/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:2829317/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:846256/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:1005319/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGat > 1:159279/1‑69 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGa > 1:2179284/1‑68 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGa > 1:2284326/1‑68 (MQ=255) aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGa > 1:1638280/1‑68 (MQ=255) ttCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa > 1:2195771/1‑69 (MQ=255) ttCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa > 1:2104636/1‑69 (MQ=255) ttCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa > 1:576752/1‑69 (MQ=255) ttCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa > 1:1839541/1‑69 (MQ=255) tCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATaa > 1:2036634/1‑69 (MQ=255) tCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATaa > 1:2683422/1‑69 (MQ=255) tCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATaa > 1:1622124/1‑69 (MQ=255) gCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa < 1:1895651/66‑1 (MQ=255) gCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa < 1:618057/66‑1 (MQ=255) ccAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATa < 1:2780941/65‑1 (MQ=255) gTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGtt < 1:2459194/69‑1 (MQ=255) gTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGtt < 1:2768206/69‑1 (MQ=255) gTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGtt < 1:176463/69‑1 (MQ=255) gTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGtt < 1:634738/69‑1 (MQ=255) gTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGtt < 1:929008/69‑1 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:507722/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:2308182/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:2505598/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:2659548/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:2660582/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:3173229/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:86194/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:1483278/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:1555313/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:1560208/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:906668/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:181078/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:1171708/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:1856036/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:688140/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:663188/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:661481/1‑69 (MQ=255) tCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa > 1:2246864/1‑69 (MQ=255) cGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1237679/69‑1 (MQ=255) cGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1313382/69‑1 (MQ=255) cGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:3195294/69‑1 (MQ=255) cGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:17665/69‑1 (MQ=255) cGTTACATTCGGGTTATTTGCCAGGGGATGATCGTCCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2077016/69‑1 (MQ=255) cGTTACATTCGGGTTATTTGCCAGGGGATGAACGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2725644/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCTAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1112298/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTTCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:541978/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt > 1:444659/1‑69 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:462517/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:908887/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:869282/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt > 1:361538/1‑69 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:481542/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:941484/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:945444/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:506166/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:949630/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:3167732/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1910520/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2022592/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2114775/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:182769/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2297266/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt > 1:1603315/1‑69 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2353497/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2476141/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2477823/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1383548/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1281816/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1226233/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:1192215/69‑1 (MQ=255) cGTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:2938946/69‑1 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:138906/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:1798458/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:1034307/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:1927720/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:1953113/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:2258474/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:60125/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:2458366/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:2571060/1‑69 (MQ=255) gTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATg > 1:2714478/1‑69 (MQ=255) gTTACACTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGAt < 1:37924/68‑1 (MQ=255) aCATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACt > 1:702683/1‑69 (MQ=255) aCATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACt > 1:2303977/1‑69 (MQ=255) aCATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACt > 1:2856961/1‑69 (MQ=255) aCATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACt > 1:3142106/1‑69 (MQ=255) | CAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACT > minE/996669‑996801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |