| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 997,014 | A→G | 73.5% | G90G (GGT→GGC) | ynfL ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 997,014 | 0 | A | G | 73.5% | 30.3 / 7.6 | 30 | G90G (GGT→GGC) | ynfL | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base G (18/4); total (22/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
AACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCGTCATCCACCATAGACAGGATTTGCCGACTATC > minE/996946‑997079 | aaCGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCa < 1:3103492/69‑1 (MQ=255) tCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGa > 1:1137425/1‑69 (MQ=255) cacCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATGCAg < 1:1355776/69‑1 (MQ=255) aTAAAAGGAGCCGACGAAGTAAAACCAATGAGCAACTCCCCCGCTTCACCCTGATGCAg < 1:1813447/59‑1 (MQ=255) aaaGGAGCGGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGa < 1:836566/69‑1 (MQ=255) aaaGGAGCGGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGa < 1:1337050/69‑1 (MQ=255) aaaGGAGCGGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGa < 1:3164572/69‑1 (MQ=255) cgacgaAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGc < 1:2057951/69‑1 (MQ=255) cgcAACTCCCCCGCTTCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCGTCATCCACCATAGACAgg > 1:989564/1‑69 (MQ=255) cgcAACTCCCCCGCTTCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCGTCATCCACCATAGACAgg < 1:3332855/69‑1 (MQ=255) cgcAACTCCCCCGCTTCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCGTCATCCACCATAGACAgg > 1:3069899/1‑69 (MQ=255) cTCCCCCGCTTCGCCCTGATGCCGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:422795/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATc > 1:828627/1‑48 (MQ=39) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATccacca > 1:2270727/1‑56 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAg > 1:1818/1‑59 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:1609707/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:1081684/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:681276/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:392083/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:3117074/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:3094159/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:1633080/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:2448391/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:2242683/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:2107816/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:2059733/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAttt > 1:1757023/1‑68 (MQ=255) cTCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAtt > 1:1227081/1‑67 (MQ=255) tCCCCCGCTTCGCCCTGATGCAGCCTTTCAGcgcgt < 1:1392683/36‑2 (MQ=38) tCCCCCGCTTCGCCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCATCATCCACCATAGACAGGAtt > 1:1460218/1‑66 (MQ=255) tCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCGTCATCCACCATAGACAGTATTTGCCGACTATc > 1:2140779/1‑68 (MQ=255) | AACGTATCGGACACCGCCCGAATAAAAGGAGCCGACGAAGTAAAACCAATGCGCAACTCCCCCGCTTCACCCTGATGCAGCCTTTCAGCGCGAGCGGCAGCGTCATCCACCATAGACAGGATTTGCCGACTATC > minE/996946‑997079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |