| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 997,128 | T→C | 100% | R52R (CGA→CGG) | ynfL ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 997,128 | 0 | T | C | 97.9% | 95.8 / ‑5.3 | 42 | R52R (CGA→CGG) | ynfL | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (40/0); total (42/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGGATTTGCCGACTATCTGCAAGAAACTGTTTTCCTGCTGCCGTCAGCAATACACTGCGATTGGTTCGTGCC > minE/997062‑997134 | cAGGATTTGCCGACTATCTGCAAGAAACTGTTTTCCTGCTGCCGTCAGCAATACACTGCGATTGGTTCg > 1:1494005/1‑69 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGt > 1:81095/1‑49 (MQ=39) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:694196/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3213693/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3308746/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3320991/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3390576/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3390791/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3394090/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3415818/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:358180/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:475383/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:640417/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:3195406/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:753367/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:77919/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:81209/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:854096/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:954233/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:968974/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:969102/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2998706/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:1210424/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:1232353/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:1531776/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:1549594/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:1654333/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2139250/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2192229/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2214871/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:223124/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:228037/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2344975/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2431441/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2460049/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2615073/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2754481/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:2911496/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:1103950/1‑52 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTACGTGc > 1:2653388/1‑51 (MQ=255) aaGAAACTGTTTTCCTGCTTCCGTCAGCAATACACTGAGATTGGTCCGTGcc > 1:290937/1‑52 (MQ=255) aaGAAAATGTTTTCCTGCTTCCGTCAGCAATACACTGCGATTGGTCCGTGcc > 1:606003/1‑52 (MQ=38) | CAGGATTTGCCGACTATCTGCAAGAAACTGTTTTCCTGCTGCCGTCAGCAATACACTGCGATTGGTTCGTGCC > minE/997062‑997134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |