BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	997,557	G→A	40.1%	Q56Q (CAG→CAA)	ynfM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	997,557	0	G	A	40.1%	58.5 / 78.9	117	Q56Q (CAG→CAA)	ynfM	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (39/31); new base A (36/11); total (75/42)
Fisher's exact test for biased strand distribution p-value = 3.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TTTATGCGCGTCACCCTGGCGCTGTTCTCTGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTCC  >  minE/997489‑997623

tttATGCGCGTCACCCTGGCGCTGTTCTCTGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAg                                                                    <  1:2526028/69‑1 (MQ=255)

            aCCCTGGCGCTGTTCTCTGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCg                                                        >  1:3373895/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:2697331/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:1621888/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:1967101/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:3338255/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:1343334/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:727720/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:731252/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:3246166/1‑69 (MQ=255)

                          ctctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGa                                          >  1:1764741/1‑69 (MQ=255)

                            ctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAg                                         <  1:1102636/68‑1 (MQ=255)

                            ctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAg                                         <  1:2712988/68‑1 (MQ=255)

                            ctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAg                                         <  1:1047539/68‑1 (MQ=255)

                            ctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAg                                         <  1:1828373/68‑1 (MQ=255)

                            ctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAg                                         <  1:2458138/68‑1 (MQ=255)

                            ctGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAg                                         <  1:2015417/68‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCCCAAGAGTTTGGCt                                  <  1:3348274/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:2745609/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:3066829/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:2040414/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:1002121/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:2410798/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:235831/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:3312241/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:503350/69‑1 (MQ=255)

                                  gACTGGCAACTTTCGCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  <  1:1493262/69‑1 (MQ=255)

                                    cTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTa                                <  1:678123/69‑1 (MQ=255)

                                    cTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTa                                <  1:2547963/69‑1 (MQ=255)

                                    cTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTa                                <  1:568068/69‑1 (MQ=255)

                                    cTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTa                                <  1:3367501/69‑1 (MQ=255)

                                    cTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTa                                >  1:526383/1‑69 (MQ=255)

                                    cTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTa                                <  1:1531382/69‑1 (MQ=255)

                                               tGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCcgcg                       >  1:1722750/1‑67 (MQ=255)

                                               tGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCcgcg                       >  1:1033373/1‑67 (MQ=255)

                                               tGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCcgcg                       >  1:501031/1‑67 (MQ=255)

                                               tGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCcgcg                       >  1:2905883/1‑67 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:2996629/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:3143182/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:3204058/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:3319515/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:3241320/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:911876/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:1006319/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:2280636/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:1785849/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:1760076/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:1968442/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAAc                    >  1:1337833/1‑69 (MQ=255)

                                                gCACTTCTCTATTGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGACTTTGGCTTAACGCCCGCGaa                     >  1:2656387/1‑68 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:1416768/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:1909582/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:3194678/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:1678886/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:1672131/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:825562/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:1267277/69‑1 (MQ=255)

                                                     tctcTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:733758/69‑1 (MQ=255)

                                                      ctctATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACagtag               <  1:103315/68‑1 (MQ=255)

                                                           ttGTTTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2227199/1‑69 (MQ=255)

                                                           ttGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTc         >  1:2745964/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCGTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:847178/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGttt                                      >  1:3238189/1‑40 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGTCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2104548/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCt                                  >  1:3051768/1‑44 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTAttt          >  1:556764/1‑68 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTAttt          >  1:254129/1‑68 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTAttt          >  1:777747/1‑68 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:1207872/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:1675805/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:914507/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2276344/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2481845/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:1930898/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:1958118/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2993245/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2562564/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2062395/1‑69 (MQ=255)

                                                           ttGTGTGCAACCTATCCTTCCGGTGCTTTCACAAGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTc         >  1:2692217/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCtt                                 >  1:2617879/1‑41 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACt      >  1:2256555/1‑68 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:3138947/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:882835/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:2159005/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:2527035/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:2157255/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:665632/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:1548838/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:2865941/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:3125851/1‑69 (MQ=255)

                                                               gtgCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTg     >  1:3159921/1‑69 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTcc  >  1:1522378/1‑69 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTcc  >  1:1612416/1‑69 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTcc  >  1:1096765/1‑69 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTcc  >  1:1817517/1‑69 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTcc  >  1:2446567/1‑69 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTc   <  1:2115582/68‑1 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTc   <  1:2783062/68‑1 (MQ=255)

                                                                  cAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTc   <  1:1022141/68‑1 (MQ=255)

                                                                  cAGCCTATCCTTACGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTc   <  1:1852525/68‑1 (MQ=255)

TTTATGCGCGTCACCCTGGCGCTGTTCTCTGCCGGACTGGCAACATTTGCACTTCTCTATTGTGTGCAGCCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACCCCCGCGAACAGTAGTATTTCACTGTCC  >  minE/997489‑997623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`