| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 999,500 | G→T | 31.5% | intergenic (+125/‑151) | asr → / → ydgD | acid shock‑inducible periplasmic protein/predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 999,500 | 0 | G | T | 31.5% | 144.0 / 70.3 | 114 | intergenic (+125/‑151) | asr/ydgD | acid shock‑inducible periplasmic protein/predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base G (38/40); new base T (17/19); total (55/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
GTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTGAT > minE/999434‑999566 | gTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat > 1:847923/1‑69 (MQ=255)gTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat > 1:267586/1‑69 (MQ=255)gTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat > 1:1969535/1‑69 (MQ=255)gTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat > 1:1345167/1‑69 (MQ=255)gTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat > 1:1811314/1‑69 (MQ=255)gTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat > 1:176303/1‑69 (MQ=255) tttAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCt < 1:1299051/69‑1 (MQ=255) tttAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCt < 1:335608/69‑1 (MQ=255) tttAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCt < 1:1703941/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:2943180/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:2320070/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:2402783/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:2571172/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:1851635/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:1644172/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:3015776/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:314663/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:1206036/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:405804/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:872637/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:3360851/69‑1 (MQ=255) tttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:574479/69‑1 (MQ=255) ttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:2866731/68‑1 (MQ=255) ttAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTTATCATCCTTATTTTATgcgc < 1:1697202/68‑1 (MQ=255) tggtggGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGtt > 1:1175021/1‑69 (MQ=255) tggtggGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGtt > 1:3374522/1‑69 (MQ=255) gtggGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGtttt > 1:3293002/1‑69 (MQ=255) gtggGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGtttt > 1:623035/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:3363154/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:1033197/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:2099631/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:681929/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:516254/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:1854965/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgcgc > 1:1609735/1‑40 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt > 1:2910555/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:3154354/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt > 1:699321/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt > 1:451442/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt > 1:2553947/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:3072220/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt > 1:1405816/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:1587425/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:1491274/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:2157636/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:2191265/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt > 1:923121/1‑69 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:1210383/69‑1 (MQ=255) aTCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:2314975/69‑1 (MQ=255) tCGCTTTGAGATCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCt < 1:3276547/68‑1 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTAcc > 1:3149795/1‑47 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:2357136/1‑69 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:1336871/1‑69 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:766296/1‑69 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:1966742/1‑69 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:2862519/1‑69 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:456138/1‑69 (MQ=255) gCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGAt > 1:592082/1‑69 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2333319/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:3344787/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:1435124/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:1472471/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:1502991/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:341546/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2340358/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2642799/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2661833/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:1658037/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2893849/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2016790/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGttt > 1:2960540/1‑52 (MQ=255) ttGAGTTCATTCTTATCATCCTTATTTTATGCGCACTGATTGCCGCCCGtt > 1:3019825/1‑51 (MQ=255) ttGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGt < 1:2753875/69‑1 (MQ=255) ttGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGt < 1:2371277/69‑1 (MQ=255) ttGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGt < 1:1408487/69‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:872087/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:314203/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:1394190/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:2912793/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:2261893/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:1641916/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:1528021/68‑1 (MQ=255) ttCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGtt < 1:785340/68‑1 (MQ=255) cATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTAt > 1:1985972/1‑68 (MQ=255) tcagATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:1043205/65‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:3436308/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:3369522/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:3134875/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:3120433/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:2837903/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:655319/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:2816986/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:2594453/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:2318775/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:2047292/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:1666620/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:1227000/68‑1 (MQ=255) tCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAATTATCTg < 1:1460870/68‑1 (MQ=255) cTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTg < 1:645198/67‑1 (MQ=255) cTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTGAt > 1:2615872/1‑69 (MQ=255) | GTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGCCCGTTTTTATCTTTCCTGATTGTAGTTATCTGAT > minE/999434‑999566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |